ACQUITY PREMIER Peptide HSS T3 Column 100Å 186009491 angle right

SKU: 186009491
ACQUITY Premier Peptide HSS T3 Column, 100Å, 1.8 μm, 2.1 x 100 mm, 1/pk

ACQUITY Premier Peptide HSS T3 Column | 186009491

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Product Description

The ACQUITY Premier Peptide HSS T3, 100Å, 1.8 µm 2.1 x 100 mm Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Each batch of HSS T3, 100Å material is QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) and must pass stringent performance criteria before acceptance for use in these columns. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods. This column is part of the MaxPeak Premier class of columns, which deliver the chromatographic performance expected from our particle technologies while increasing reproducibility, peak shape, and recovery for metal-sensitive analytes.

Suggested Use: Peptide Separations, Peptide Analysis

Specifications

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    2 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    45 C

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Bonding Technology

    T3

  • Silanol Activity

    Medium

  • Particle Shape

    Spherical

  • Particle Size

    1.8 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    100 Å

  • QC Tested

    Peptide

  • Format

    Premier Column

  • System

    UHPLC, UPLC

  • Particle Technology

    HSS

  • Technique

    LC

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    100 mm

  • Carbon Load

    11 %

  • eCord

    Yes

  • UNSPSC

    41115711

  • Application

    Peptide

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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ACQUITY Premier Peptide HSS T3 Column, 100Å, 1.8 μm, 2.1 x 100 mm, 1/pk

A universal, silica-based bonded phase is employed in the sorbent of the ACQUITY Premier Peptide HSS T3 Column and is compatible with a 100% aqueous mobile phase. Your primary option for the retention and separation of polar and non-polar chemicals should be dependable lab equipment. The ACQUITY Premier Peptide HSS T3 Column is completely scalable and transferrable to HPLC separations, and it exhibits extremely little MS bleed.

The trifunctional C18 alkyl phase is bonded at a ligand density that encourages polar compound retention and compatibility with aqueous mobile phases as part of the advanced T3 bonding process, which is used in the production of the ACQUITY Premier Peptide HSS T3 Column. The patented T3 end-capping technology is far more efficient than the conventional trimethyl silane (TMS) end-capping. In comparison to conventional columns, this novel end-capping with T3 bonding offers superior polar compound retention and water compatibility, as well as improved lab equipment performance, longevity, peak shape, and stability. Reversed-phase HPLC and UPLC technologies are used by the ACQUITY Premier Peptide HSS T3 Column to keep its position as the market leader.

The MaxPeakTM High Performance Surfaces, a cutting-edge technology used in the ACQUITY Premier Peptide HSS T3 Column, has been developed to improve analyte recovery, sensitivity, and reproducibility by reducing analyte/surface interactions that can result in sample losses and unsatisfactory peak shape.

The ACQUITY Premier Peptide HSS T3 Column is produced in a cGMP, ISO 9001-certified facility utilizing ultra-pure reagents and packaging materials created exclusively for use with the ACQUITY UPLC System. Each batch of ACQUITY packing material is further tested chromatographically for quality control using neutral, acidic, and basic analytes and the results are held to precise specification ranges to ensure great reproducible performance. A Performance Chromatogram and Certificate of Batch Analysis are delivered on the eCord Intelligent Chip along with the column for your reference after each column has undergone individual testing.

You may also be interested in the mAb Tryptic Digestion Standard.

What Is The ACQUITY Premier Peptide HSS T3 Column’s pH Range?

The Waters ACQUITY Premier Peptide HSS T3 Column listed here is able to work well within the 2-8 pH range.

What Is The Recommended Maximum Pressure That The ACQUITY Premier Peptide HSS T3 Column Can Tolerate?

For the ACQUITY Premier Peptide HSS T3 Column, Waters recommends a maximum of 18000 psi (1240 Bar) pressure.