Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
QC Tested |
Oligonucleotide |
Format |
Premier Column |
System |
UPLC |
Particle Technology |
BEH |
Technique |
LC |
USP Classification |
L1 |
Inner Diameter |
2.1 mm |
Length |
150 mm |
Carbon Load |
18 % |
eCord |
Yes |
UNSPSC |
41115711 |
Application |
Oligonucleotide, Gene Therapeutics |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY Premier Oligonucleotide C18 Column, 130Å, 1.7 µm, 2.1 x 150 mm, 1/pk
ACQUITY Premier Oligonucleotide Columns are used in reversed-phase chromatography to separate detritylated synthetic oligonucleotides. Waters MassPrep OST standards are used to QC each column to ensure repeatable performance for your most demanding oligonucleotide assays. This column belongs to the MaxPeak Premier Class of Columns, which provide the chromatographic performance expected of our particle technologies while improving reproducibility, peak shape, and recovery for metal-sensitive analytes.
The ACQUITY Premier Oligonucleotide C18 Column, which belongs to the MaxPeak Premier family of columns, uses the MaxPeak Premier features to provide you with reduced column conditioning and passivation times, as well as simpler mobile phases without complex additives. The ACQUITY Premier Oligonucleotide C18 Column, when combined with the tri-functionally bonded BEH particle technology, will save you time in method development while providing exceptional peak shape for basic analytes at elevated pH, stability across a pH range of 1 - 12 pH and high temperature.
The ACQUITY Premier Oligonucleotide C18 Column, like all Waters lab equipment, is made with ultrapure reagents, ensuring control of the chemical composition and purity of the hybrid particle. Every step of the manufacturing process is meticulously monitored and controlled within extremely tight tolerances. Waters MassPrep OST standards are used to QC each column to ensure repeatable performance for your most demanding oligonucleotide assays. Certificates of Batch Analysis and Column Efficiency are provided with each column to ensure that you are working with high-quality machinery. Waters manufactures dependable laboratory equipment that allows you to achieve repeatable results batch after batch. All Water plants and facilities adhere to cGMP and ISO 9001 standards.
By using our website or browsing through our brochure, you can shop for lab equipment to meet the demanding needs of your laboratory. We also recommend you to check out MassPREP Oligonucleotide Standard; The MassPREPTM Oligonucleotide Separation Technology (OST) Standard is intended for testing the performance of HPLC/UPLC instruments and columns for the analysis of synthetic oligonucleotides. Lyophilized and packaged in 1.5 ml LC vials are equimolar amounts of 15, 20, 25, 30, and 35 nucleotide (nt) long oligo-deoxythymidine. The shipped vial is vacuum-sealed in foil pouches to reduce degradation caused by excessive light and air exposure.
What Is the Carbon Load Capacity of the ACQUITY Premier Oligonucleotide C18 Column?
The ACQUITY Premier Oligonucleotide C18 Column has a carbon load of 18%, a particle size of 1.7m, and a pore size of 130. It can also withstand a maximum pressure of 18000 pounds per square inch (or 1240 Bar).