SKU: 186009265
nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 300 µm X 150 mm, 1/pk

nanoEase M/Z Peptide BEH C18 Column | 186009265


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Product Description

nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 300 µm X 150 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. The 300µm ID columns are built utilizing a ultra low dispersion column design and offer the same simplicity of use as more traditional columns. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 300Å pore, C18, 1.7 µm particles. The wide-pore (300Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for especially large peptides in proteomics, protein characterization, and peptide synthesis. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) to before being QC accepted as a Peptide separation column.

Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    12 pH

  • Maximum Pressure

    15000 psi (1034 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    300 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    90

  • System

    Nano/Micro

  • Particle Technology

    BEH

  • USP Classification

    L1

  • Inner Diameter

    300 µm

  • Length

    150 mm

  • Carbon Load

    12 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    nanoEase M/Z

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 300 µm X 150 mm, 1/pk

The Waters nanoEase™ M/Z columns are designed for optimal performance for nano- to micro-scale liquid chromatographic separation, offering the highest possible sensitivity, resolution, and reproducibility for biomarker discovery and protein identification and characterization. The 300µm ID columns are built utilizing a ultra-low dispersion column design and offer the same simplicity of use as more traditional columns. Like all M/Z columns, they withstand high operating pressure and handle pressure fluctuations during injection extremely well and thus providing superior longevity.

In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 300A pore, C18, 1.7 um particles, and each batch is specifically QC-tested with a carefully selected protein standard mix to help ensure consistent performance and increased analytical column life.

The ZenFit Connectors included with the nanoEase M/Z Peptide BEH C18 Column are intended for use with V-detail style connection ports. The V-detail connection port is a 10-32 threaded port for 1/16" O.D. tubing that is simple to use, reusable, and fingertight. It can withstand pressures of up to 90°C. The procedure of establishing critical nano- and microflow UPLC fluid connections is simplified as a result. You may significantly reduce flow-path-induced variability in chromatographic findings using the nanoEase M/Z Peptide BEH C18 Column.

The nanoEase M/Z Peptide BEH C18 packing materials were designed specifically for use with the ACQUITY UPLC System and are manufactured in a cGMP, ISO 9001-certified facility using ultra-pure reagents. Each batch of nanoEase M/Z Peptide BEH C18 packing material is chromatographically tested with acidic, basic, and neutral analytes, with the results constrained to narrow specification ranges to assure exceptional, repeatable performance. Every column is analyzed individually, and a Performance Chromatogram and a Certificate of Batch Analysis are generated using an eCordTM Intelligent Chip.

Search our website and shop for lab equipment any time, such as the Atlantis Premier BEH Z-HILIC VanGuard FIT Column, 5 µm, 2.1 mm X 50 mm, 1/pk which may be helpful alongside your other pieces of equipment.

What pH Range Does The nanoEase M/Z Peptide BEH C18 Column Offer?

The nanoEase M/Z Peptide BEH C18 Column listed here is designed to offer a broad operating pH range of 1-12 pH.

What Is The Maximum Pressure That The nanoEase M/Z Peptide BEH C18 Column Can Tolerate?

The nanoEase M/Z Peptide BEH C18 Column can work with high pressures and can sustain up to 15000 psi (1034 Bar) pressure.