Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
185 |
System |
Nano/Micro |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
300 µm |
Length |
50 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC M-Class |
Product Type |
Columns |
Units per Package |
1 pk |
nanoEase M/Z Peptide BEH C18 Column, 130Å, 1.7 µm, 300 µm X 50 mm, 1/pk
For best performance in nano- to micro-scale liquid chromatographic separation, rely on the nanoEase M/Z Peptide BEH C18 Column. For biomarker discovery and protein identification and characterization, the lab equipment provides the best possible sensitivity, resolution, and repeatability.
Waters' patented BEH Technology 130A pore, C18, 1.7 µm particles are used in the nanoEase M/Z Peptide BEH C18 Column version shown below. This aids in achieving optimal performance by ensuring adequate connections are made to avoid peak tailing and inefficient operation.
Each batch of the nanoEase M/Z Peptide BEH C18 Column is QC tested using a gradient separation of a tryptic digest of cytochrome c before being QC accepted as a Peptide separation column to ensure you receive only the highest quality lab equipment.
Created expressly for use with the ACQUITY UPLC System and produced in a cGMP, ISO 9001-certified facility using ultra-pure reagents, the nanoEase M/Z Peptide BEH C18 packing materials are made to the highest standards. Each batch of nanoEase M/Z Peptide BEH C18 packing material is chromatographically tested with acidic, basic, and neutral analytes, with the results confined to tight specification ranges to ensure outstanding, repeatable performance. Every column is individually examined, and an eCordTM Intelligent Chip is used to give a Performance Chromatogram and a Certificate of Batch Analysis.
The ZenFit Connectors that come pre-assembled with the nanoEase M/Z Peptide BEH C18 Column are designed to be utilized in V-detail style connection ports. The V-detail connection port is a 10-32 threaded port for 1/16" O.D. tubing that is easy to use, reusable, and fingertight and can resist pressures at 90°C. This simplifies the process of establishing essential nano- and microflow UPLC fluid connections. With the nanoEase M/Z Peptide BEH C18 Column, you can considerably eliminate flow-path-induced variability in chromatographic findings.
The nanoEase M/Z Peptide BEH C18 Column has an ultra-low dispersion design to provide the same ease of use as more standard columns. The nanoEase M/Z Peptide BEH C18 Column, like other lab equipment with comparable chemistries, can withstand high operating pressures and handle pressure fluctuations during injection exceedingly well, resulting in an enhanced lifetime. Utilize alongside other Waters’ products, such as the nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 75 µm X 200 mm, 1/pk.
What pH Range Is Offered By The nanoEase M/Z Peptide BEH C18 Column?
The nanoEase M/Z Peptide BEH C18 Column offers a 1-12 pH range.