SKU: 186009257
nanoEase M/Z Peptide BEH C18 Column, 130Å, 1.7 µm, 300 µm X 50 mm, 1/pk

nanoEase M/Z Peptide BEH C18 Column | 186009257


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Product Description

nanoEase M/Z Peptide BEH C18 Column, 130Å, 1.7 µm, 300 µm X 50 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. The 300 µm ID columns are built utilizing a ultra low dispersion column design and offer the same simplicity of use as more traditional columns. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 130Å pore, C18, 1.7 µm particles. The small-pore (130Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) to before being QC accepted as a Peptide separation column.

Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    12 pH

  • Maximum Pressure

    15000 psi (1034 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    185

  • System

    Nano/Micro

  • Particle Technology

    BEH

  • USP Classification

    L1

  • Inner Diameter

    300 µm

  • Length

    50 mm

  • Carbon Load

    12 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    ACQUITY UPLC M-Class

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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nanoEase M/Z Peptide BEH C18 Column, 130Å, 1.7 µm, 300 µm X 50 mm, 1/pk

For best performance in nano- to micro-scale liquid chromatographic separation, rely on the nanoEase M/Z Peptide BEH C18 Column. For biomarker discovery and protein identification and characterization, the lab equipment provides the best possible sensitivity, resolution, and repeatability.

Waters' patented BEH Technology 130A pore, C18, 1.7 µm particles are used in the nanoEase M/Z Peptide BEH C18 Column version shown below. This aids in achieving optimal performance by ensuring adequate connections are made to avoid peak tailing and inefficient operation.

Each batch of the nanoEase M/Z Peptide BEH C18 Column is QC tested using a gradient separation of a tryptic digest of cytochrome c before being QC accepted as a Peptide separation column to ensure you receive only the highest quality lab equipment.

Created expressly for use with the ACQUITY UPLC System and produced in a cGMP, ISO 9001-certified facility using ultra-pure reagents, the nanoEase M/Z Peptide BEH C18 packing materials are made to the highest standards. Each batch of nanoEase M/Z Peptide BEH C18 packing material is chromatographically tested with acidic, basic, and neutral analytes, with the results confined to tight specification ranges to ensure outstanding, repeatable performance. Every column is individually examined, and an eCordTM Intelligent Chip is used to give a Performance Chromatogram and a Certificate of Batch Analysis.

The ZenFit Connectors that come pre-assembled with the nanoEase M/Z Peptide BEH C18 Column are designed to be utilized in V-detail style connection ports. The V-detail connection port is a 10-32 threaded port for 1/16" O.D. tubing that is easy to use, reusable, and fingertight and can resist pressures at 90°C. This simplifies the process of establishing essential nano- and microflow UPLC fluid connections. With the nanoEase M/Z Peptide BEH C18 Column, you can considerably eliminate flow-path-induced variability in chromatographic findings.

The nanoEase M/Z Peptide BEH C18 Column has an ultra-low dispersion design to provide the same ease of use as more standard columns. The nanoEase M/Z Peptide BEH C18 Column, like other lab equipment with comparable chemistries, can withstand high operating pressures and handle pressure fluctuations during injection exceedingly well, resulting in an enhanced lifetime. Utilize alongside other Waters’ products, such as the nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 75 µm X 200 mm, 1/pk.

What pH Range Is Offered By The nanoEase M/Z Peptide BEH C18 Column?

The nanoEase M/Z Peptide BEH C18 Column offers a 1-12 pH range.