SKU: 186009256
nanoEase M/Z Peptide CSH C18 Column, 130Å, 1.7 µm, 300 µm X 150 mm, 1/pk

nanoEase M/Z Peptide CSH C18 Column | 186009256


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Product Description

nanoEase M/Z Peptide CSH C18 Column, 130Å, 1.7 µm, 300 µm X 150 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. The 300 µm ID columns are built utilizing a ultra low dispersion column design and offer the same simplicity of use as more traditional columns. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented CSH Technology 130Å pore, C18, 1.7 µm particles. The first choice for peptide separations, CSH particles contain a low and carefully-defined concentration of positive charges that yield comparatively excellent peak shape for peptides using either FA- or TFA-containing mobile phases. They exhibit little dependence on strong ion pairing agents, making them ideal for LC or LC-MS applications. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) to before being QC accepted as a Peptide separation column.

Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    11 pH

  • Maximum Pressure

    15000 psi (1034 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    185

  • System

    Nano/Micro

  • Particle Technology

    CSH

  • USP Classification

    L1

  • Inner Diameter

    300 µm

  • Length

    150 mm

  • Carbon Load

    15 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    nanoEase M/Z

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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nanoEase M/Z Peptide CSH C18 Column, 130Å, 1.7 µm, 300 µm X 150 mm, 1/pk

Depend on the nanoEase M/Z Peptide CSH C18 Column to get optimal performance for nano- to micro-scale liquid chromatographic separation. The lab equipment offers the highest possible sensitivity, resolution, and reproducibility for biomarker discovery and protein identification and characterization.

Built utilizing an ultra-low dispersion design, the nanoEase M/Z Peptide CSH C18 Column offers the same simplicity of use as more traditional columns. Like other lab equipment of similar chemistries, the nanoEase M/Z Peptide CSH C18 Column can tolerate operating pressure of up to 15,000 psi and handle pressure fluctuations during injection extremely well, resulting in superior longevity.

The nanoEase M/Z Peptide CSH C18 Column variant listed here contains Waters' patented BEH Technology 130A pore, C18, 1.7um particles. This helps you achieve maximum performance, as it ensures that proper connections are made to minimize peak tailing and poor efficiency.

To make sure you receive only the best quality lab equipment, each batch of the nanoEase M/Z Peptide CSH C18 Column is QC tested using a gradient separation of a tryptic digest of cytochrome c before being QC accepted as a Peptide separation column.

The nanoEase M/Z Peptide CSH C18 Column comes pre-assembled with ZenFit Connectors which are designed to be used in V-detail style connection ports. The V-detail connection port is a 10-32 threaded port for 1/16” O.D. tubing, which are easy-to-use, reusable, fingertight connections, that can withstand 15,000 psi at 90 °C. This introduces great simplicity in creating critical nano- and microflow UPLC fluid connections. You can greatly reduce the flow-path-induced variability in chromatographic results with the nanoEase M/Z Peptide CSH C18 Column.

Find all the variants of the nanoEase M/Z Peptide CSH C18 Column and products that work in conjunction with the column. Shop for lab equipment, and learn more about our brands and technologies through our website while adding to your lab equipment list.

You may also be interested in the nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 300 µm X 50 mm, 1/pk.

What Safety Precautions Are Recommended?

Always use appropriate safety glasses or goggles that meet the ANSI Z87.1-1989 requirements when handling fused silica tubing.