Line drawing of a column

SKU: 186009127
XBridge Protein BEH C4 Column, 300Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk

XBridge BEH C4 Column | 186009127


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Product Description

The XBridge Protein BEH C4 Column, 300Å, 2.5 µm, 2.1 mm X 50 mm columns are optimized and QC tested with the MassPREP Protein Mix standard (p/n: 186004900) for protein separations. The 300Å pore size trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed for various protein based applications.

Suggested Use: Suggested use: Low-level intact protein and subunit analysis

If you are developing a new method, please consider our newer technology, BioResolve RP mAb Polyphenyl, XBridge Premier Protein BEH C4 and ACQUITY Premier Protein BEH C4 Columns for superior separation, speed, recovery, and resolution.

Specifications

  • Chemistry

    C4

  • Temperature Limits

    90 C

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Particle Size

    2.5 µm

  • Pore Size

    300 Å

  • QC Tested

    Protein

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L26

  • Inner Diameter

    2.1 mm

  • Length

    50 mm

  • UNSPSC

    41115709

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XBridge BEH C4 Column, 300Å, 2.5 µm, 2.1 mm X 50 mm, 1/pk

The XBridge Protein BEH C4 Column provides excellent peptide separation for a wide range of peptides. For maximum resolution, it also produces thin and symmetrical peaks. The XBridge Protein BEH C4 Column provides excellent peak shape and retention in formic acid and trifluoroacetic acid for optimum chromatography.

To assure the stability of peptide separation procedures, the XBridge Protein BEH C4 Column is QC verified with a peptide map. This is made achievable by using well-characterized, cutting-edge C18 ligand bonding techniques. Furthermore, it ensures consistent results with a wide range of samples utilized in proteomics, protein characterization, and peptide synthesis. The analytical column that results provides consistent synthetic peptide and protein digest separations from batch to batch. The lab equipment's manufacturing procedures enable stable particle structure and bonding chemistry pH at elevated temperatures.

Benefit from the Protein Separation Technology of the XBridge Protein BEH C4 Column, which includes BEH technology and uses synthetic particles to provide the best quality and consistent performance. For biological macromolecules that are too big or hydrophobic for separation on columns with smaller pores or longer chain bonded phases, the XBridgeTM BEH C4, 300 Å packing material was intended to give excellent peak shape, high efficiency, and good recovery. The base particle and bonding chemistry have been selected to give outstanding stability at high and low pH levels, as well as at high temperatures.

The XBridge BEH C4 Column packing materials were created especially for use with the ACQUITY UPLC System and are made in a cGMP, ISO 9002 certified facility using ultra-pure reagents. Each batch of XBridge BEH C4 Column packing material is chromatographically tested with acidic, basic, and neutral analytes, with results held within narrow specification ranges to ensure excellent consistent performance. On the eCordTM Intelligent Chip, each column is carefully examined, and a Performance Chromatogram and Certificate of Batch Analysis are provided. Find the complete range of XBridge columns and shop for lab equipment using our online store.

You may also wish to consider the XBridge Premier BEH C18 VanGuard FIT Column, 130Å, 2.5 µm, 4.6 x 150 mm, 1/pk. It can be used alongside this product and is often used in conjunction with other similar products manufactured by Waters.

What Is The Recommended pH For The XBridge BEH C4 Column?

The recommended operating pH range for XBridge BEH C4, 300 Å Column listed here is 2 to 10 pH.