Line drawing of a column

SKU: 186008964
XBridge Oligonucleotide BEH C18 OBD Prep Column, 130Å, 2.5 µm, 50 mm X 50 mm, 1/pk

XBridge Oligonucleotide BEH C18 OBD Prep Column | 50 mm X 50 mm | 1/pk | 186008964

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Product Description

Waters Oligonucleotide Separation Technology columns are based on the second generation of hybrid-silica BEH Technology particles functionalized with C18. The separation of detritylated synthetic oligonucleotide samples is based on the well-established method of ion-pair, reversed-phase chromatography. The availability of 1.7µm UPLC technology or 2.5µm HPLC particles in various column dimensions provides flexibility to meet various laboratory-scale isolation or analysis needs and delivers exceptional sample resolution and superior column life. In addition, Waters manufacturing and quality control testing procedures help ensure consistent batch-to-batch and column-to-column performance regardless of application demands.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Size

    2.5 µm

  • Pore Size

    130 Å

  • Format

    OBD Prep Column

  • Particle Technology

    BEH

  • USP Classification

    L1

  • Inner Diameter

    50 mm

  • Length

    50 mm

  • UNSPSC

    41115854

  • Application

    Oligonucleotide, Gene Therapeutics

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk
Hazaradous Material This product contains hazardous material which requires special freight handling. Additional charges may apply.

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XBridge Oligonucleotide BEH C18 OBD Prep Column, 130Å, 2.5 µm, 50 mm X 50 mm, 1/pk

Maximize your productivity with the higher recoveries offered by the Waters XBridge Oligonucleotide BEH C18 OBD Prep Column. The lab equipment also offers longer column lifetimes, requiring fewer replacements and, therefore, reducing costs as well.

Attain more efficiency through the Optimum Bed Density (OBD) preparative columns. They enable you to achieve lab-scale separations for greater throughput in a faster, more efficient manner. With the XBridge Oligonucleotide BEH C18 OBD Prep Column, you also have the ability to directly scale from UPLC or HPLC screening to lab-scale purification. The Waters patented OBD preparative column design and packing process ensures that you get predictable, uniform density profiles throughout the column. There is no potential for voids because during the capping process, there is no overcompression or disruption in any non-uniform way.

The Waters XBridge Oligonucleotide BEH C18 OBD Prep Column offers exceptional resistance to mechanical chromatographic bed failure by leveraging the OBD design that is resistant to poor solubility and pressure shocks associated with large volumes of pure organic solvent. You also get consistent column-to-column performance, reducing the costs through extended lifetimes. This is made possible by adhering to the strictest manufacturing conditions such as the cGMP, ISO 9001:2000 using ultra-pure reagents.

You may also want to explore other variations of the Waters XBridge Oligonucleotide BEH C18 OBD Prep Column such as the XBridge Oligonucleotide BEH C18 OBD Prep Column, 130Å, 2.5 µm, 30 mm X 50 mm, 1/pk. See our complete portfolio including the complete range of Waters Xbridge BEH Columns and shop for lab equipment as well.

How Is Column Efficiency Determined In The XBridge Oligonucleotide BEH C18 OBD Prep Column?

To determine the initial column efficiency, you will need to conduct an efficiency test on the column prior to using it in the desired application. It is recommended that a suitable mixture, as found in the “Performance Test Chromatogram”, be used to analyze the column. Determine the number of theoretical plates and use this value for periodic comparisons. To ensure that the column is still performing at its optimum efficiency, repeat the test at predetermined intervals. Quality of connections may give slight variations on different HPLC systems. Other factors that may impact the efficiency and results include operating environment, system electronics, reagent quality, and operator technique.