Line drawing of a column

SKU: 186008949
BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 3 mm X 100 mm, 1/pk

BioResolve RP mAb Polyphenyl Column | 186008949

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Product Description

The first choice for reverse phase protein separation and analysis, BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 3 mm X 100 mm Columns contain 2.7 µm superficially-porous-silica particles having an average pore diameter of 450Å. The particles are functionalized with a proprietary polyphenyl bonding process that was specifically designed for LC or LC-MS analyses of intact IgG monoclonal antibodies and their fragments. Each batch is qualified using a gradient separation of the Waters mAb Subunit Standard (p/n: 186008927).

Note: p/n: 176004163 contains this same column with Intact mAb Mass Check Standard (p/n: 186006552) and mAb Subunit Standard (p/n: 186008927).

Suggested Use: Intact protein and mAb subunit analysis, Protein Analysis, ADC characterization, AAV, Antibody Conjugates

Specifications

  • Separation Mode

    Reversed Phase

  • Temperature Limits

    90 C

  • Maximum Pressure

    10000 psi (689 Bar)

  • Particle Size

    2.7 µm

  • Pore Size

    450 Å

  • QC Tested

    Protein

  • System

    HPLC, UHPLC, UPLC

  • USP Classification

    L11

  • UNSPSC

    41115709

  • Brand

    BioResolve

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents

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BioResolve RP mAb Polyphenyl Column, 450Å, 2.7 µm, 3 mm X 100 mm, 1/pk

Specific analytical challenges apply to the analysis of mAb-based and associated biotherapeutic drugs, so don’t you want a chromatography column that can overcome all of those challenges?
With the BioResolve RP mAb Polyphenyl Column, you get exactly that and so much more. At the heart of this monoclonal antibody analysis column is an innovative solution for the intact protein analysis of mAbs and ADCs. But even more, this column provides a wide and usable pH range to accommodate varying analytes. Improve your monoclonal antibody quantitation workflow with regards to reverse-phase analysis by purchasing the BioResolve RP mAb Polyphenyl Column today.

To provide an innovative solution for monoclonal antibody analysis, the BioResolve RP mAb Polyphenyl Column was designed using a novel phenyl bonding process that makes this column provide better chromatographic resolution at higher flow rates. And since this column can be used on all UHPLC, UPLC and HPLC systems, that resistance to increased flow rates is greatly appreciated by scientists across the globe. It’s the goal of the BioResolve RP mAb Polyphenyl Column to maximize selectivity and the resolving power of various analyzed protein samples, and it does so with flying colors and a rigidity that makes this column resistant to overwhelming chromatographic conditions.

Another major benefit to owning the BioResolve RP mAb Polyphenyl Column is that it provides minimal injection-to-injection carryover for increased confidence in analyses. This is because the high-coverage bonding prevents carryover effects and increases low pH stability for improved column lifetime. And if you’re concerned about overall column performance, you don’t have to be, as the BioResolve RP mAb Polyphenyl Column must undergo strict QC testing with NIST-based mAb standard before being packed and shipped to you.

Speaking of overall column lifetimes, did you know that adding a VanGuard column protection solution to your workflow can protect the terrific performance provided by the BioResolve RP mAb Polyphenyl Column?
Simply add the BioResolve RP mAb Polyphenyl VanGuard Cartridge to your analytical workflow and you can better protect your precious separations from variability caused by particulate contaminants.

Before checking out with your BioResolve RP mAb Polyphenyl Column, shop lab equipment to explore all of the other incredible products, services and resources that Waters has to provide. It’s our passion to make your lab more efficient and your assays more successful with our offerings.

How Can I Test the BioResolve RP mAb Polyphenyl Column for Performance?

The BioResolve RP mAb Polyphenyl Column can be tested for performance using the Intact mAb Mass Check Standard (P/N 186006552) and mAb Subunit Standard (P/N 186008927).

Are There Any Temperature Limits For The BioResolve RP mAb Polyphenyl Column?

At temperatures between 50 and 90 °C, BioResolve RP mAb Polyphenyl Columns are advised for usage. A lower temperature separation should be preferred whenever possible. In order to retain desired results, it is advised to start developing a new separation process at a relatively high temperature of 80 to 90 °C and then reduce it to as low as 50 °C, depending on the composition of the mAb.