SKU: 186008815
nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 75 µm X 100 mm, 1/pk

nanoEase M/Z HSS T3 Column | 186008815


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Product Description

nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 75 µm X 100 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. The 300 µm ID columns are built utilizing a ultra low dispersion column design and offer the same simplicity of use as more traditional columns. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters HSS T3, 100Å pore, 1.8 µm particles and is designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. The columns are equipped with the ZenFit connector, an easy to use, fingertight, tool free liquid connection. This ensures reproducible, leak free connections. The fittings will fit into Waters V-Detail ports as well as into Rheodyne/Vici compatible valves.

Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    2 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    45 C

  • Maximum Pressure

    15000 psi (1034 Bar)

  • Endcapped

    Yes

  • Bonding Technology

    T3

  • Silanol Activity

    Medium

  • Particle Shape

    Spherical

  • Particle Size

    1.8 µm

  • Endfitting Type

    10-32 for 1/16" male /360um open

  • Pore Size

    100 Å

  • Format

    Column

  • Surface Area

    230

  • System

    Nano/Micro

  • Particle Technology

    HSS

  • USP Classification

    L1

  • Inner Diameter

    75 µm

  • Length

    100 mm

  • Carbon Load

    11 %

  • UNSPSC

    41115711

  • Brand

    nanoEase M/Z

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 75 µm X 100 mm, 1/pk

For the best results in liquid chromatographic separation at the nano- to microscale, rely on the nanoEase M/Z HSS T3 Column. The analytical column provides the highest level of sensitivity, resolution, and repeatability for the identification and characterization of proteins and the discovery of biomarkers. This particular lab equipment has a 75 µm ID that offers the same ease of use as more conventional columns and is constructed using an extremely low dispersion column design. It is made to endure operating pressures of 15 kpsi (1,000 bar) and handle pressure changes during injection quite effectively. It offers superior column longevity as a result.

ZenFit Connectors are pre-assembled on the nanoEase M/Z HSS T3 Column. The V-detail type connector ports are where ZenFit Connectors are intended to be used. The 10-32 threaded port used for the V-detail connection may accommodate 1/16" O.D. tubing. These fingertight couplings are simple to use, reusable, and capable of withstanding high pressures and temperatures. This makes crucial nano- and microflow UPLC fluid connections quite simple to create. The nanoEase M/Z HSS T3 Column significantly reduces the flow-path-caused variability in chromatographic findings. The nanoEase M/Z HSS T3 Column is the appropriate lab equipment due to all of these characteristics.

Special care is given throughout the production process to produce the highest standards since it is crucial to make sure that suitable connections are made to reduce peak tailing and inefficient operation in order to achieve optimal performance. Before being QC recognized as a Peptide separation column, each batch is QC evaluated utilizing a gradient separation of a tryptic digest of cytochrome c.

Refer to our catalog to learn more about our technology, purchase lab equipment, and browse our whole selection of products as well as all connected items and other listing variations.

You may also want to explore the nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 300 µm X 150 mm, 1/pk.

What Is The pH Range Offered By The Waters nanoEase M/Z HSS T3 Column?

The Waters nanoEase M/Z HSS T3 Column listed here is able to function across the 2-8 pH range.

What Are The Temperature Limits Of The nanoEase M/Z HSS T3 Column?

This particular Waters nanoEase M/Z HSS T3 Column can sustain a maximum of 45 C temperature. However, this may be affected by the varying buffers, pH, and pressure limits.