SKU: 186008806
nanoEase M/Z Protein BEH C4 Column, 300Å, 1.7 µm, 150 µm X 100 mm, 1/pk

nanoEase M/Z Protein BEH C4 Column | 186008806

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Product Description

Waters nanoEase M/Z Protein BEH C4 Column, 300Å, 1.7 µm, 150 µm X 100 mm Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All nanoLC columns are designed for use up to 15 kpsi (1,000 Bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.The columns are equipped with the ZenFit connector, an easy to use, fingertight, tool free liquid connection. This ensures reproducible, leak free connections. The fittings will fit into Waters V-Detail ports as well as into Rheodyne/Vici compatible valves. This specific offering contains Waters patented BEH Technology 300Å pore, C4, 1.7 µm particles. These particles are QC tested with the MassPREP Protein Mix standard (p/n: 186004900) and are optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.

Suggested Use: Top-down Proteomics, Nano-flow, Trap and Elute

Specifications

  • Chemistry

    C4

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    10 pH

  • Maximum Pressure

    15000 psi (1034 Bar)

  • Endcapped

    No

  • Silanol Activity

    Medium

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    10-32 for 1/16" male /360um open

  • Pore Size

    300 Å

  • QC Tested

    Protein

  • Format

    Column

  • Surface Area

    90

  • System

    Nano/Micro

  • Particle Technology

    BEH

  • USP Classification

    L26

  • Inner Diameter

    150 µm

  • Length

    100 mm

  • Carbon Load

    8 %

  • UNSPSC

    41115711

  • Application

    Protein

  • Brand

    nanoEase M/Z

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

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nanoEase M/Z Protein BEH C4 Column, 300Å, 1.7 µm, 150 µm X 100 mm, 1/pk

For the best outcomes in liquid chromatographic separation at the nano- to microscale, use the nanoEase M/Z Protein BEH C4 Column. For the identification and characterization of proteins as well as the finding of biomarkers, lab equipment has the best sensitivity, resolution, and repeatability.

The version of the nanoEase M/Z Protein BEH C4 Column that is shown here makes use of the exclusive 300A pore, C4, 1.7 µm particles from Waters. This contributes to achieving optimal performance by ensuring that the proper connections are made to prevent peak tailing and wasted operation.

The nanoEase M/Z Protein BEH C4 Column packing materials were made in a cGMP, ISO 9001-certified laboratory using ultra-pure reagents and were created expressly for use with the ACQUITY UPLC System. Each batch of nanoEase M/Z Protein BEH C4 Column packing material is chromatographically tested with acidic, basic, and neutral analytes to ensure superior, consistent performance. The findings are limited to specified ranges of values. After each column has been independently examined, an eCord Intelligent Chip is employed to provide a Performance Chromatogram and a Certificate of Batch Analysis.

Each batch of the nanoEase M/Z Protein BEH C4 Column is QC tested using a gradient separation of a tryptic digest of cytochrome c to ensure that you only get the best lab equipment. The pre-assembled ZenFit Connectors for the nanoEase M/Z Protein BEH C4 Column are designed to fit into connection ports with a V-detail appearance. The 10-32 threaded V-detail connection port is made for 1/16" O.D. tubing, is fingertight, and is reusable. It can endure 90 °C pressures. As a result, critical connections for nano- and microflow UPLC fluids are made simpler. Flow-path-induced variability in chromatographic findings may be successfully minimized with the nanoEase M/Z Protein BEH C4 Column.

To fulfill your various chromatographic needs, the Waters nanoEase columns are offered in a wide range of configurations. You may also want to explore the nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 75 µm X 200 mm, 1/pk.

Across What pH Range Does The nanoEase M/Z Protein BEH C4 Column?

The Waters nanoEase M/Z Protein BEH C4 Column listed here is able to work well across the 1 pH - 10 pH range.

What Is The Maximum Pressure That The nanoEase M/Z Protein BEH C4 Column Can Sustain?

The Waters nanoEase M/Z Protein BEH C4 Column is able to tolerate a maximum of 15,000 psi (1034 Bar) pressure.