Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
10 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
10-32 for 1/16" male /360um open |
Pore Size |
300 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
90 |
System |
Nano/Micro |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
75 µm |
Length |
250 mm |
Carbon Load |
12 % |
UNSPSC |
41115711 |
Application |
Peptide |
Brand |
nanoEase M/Z |
Product Type |
Columns |
Units per Package |
1 pk |
nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 75 µm X 250 mm, 1/pk
Use the nanoEase M/Z Peptide BEH C18 Column for the best results in liquid chromatographic separation at the nano- to microscale. The lab equipment offers the best sensitivity, resolution, and repeatability for biomarker discovery and protein identification and characterization. The nanoEase M/Z Peptide BEH C18 Column version below uses Waters' proprietary 300 A pore, C18, 1.7 µm particles. Making sure appropriate connections are made to prevent peak tailing and wasteful operation helps to achieve optimal performance.
In order to guarantee that you only receive the best lab equipment, each batch of the nanoEase M/Z Peptide BEH C18 Column is QC verified utilizing a gradient separation of a tryptic digest of cytochrome c.
The nanoEase M/Z Peptide BEH C18 packing materials were developed specifically for use with the ACQUITY UPLC System and are manufactured in a cGMP, ISO 9001-certified facility using ultra-pure reagents. Each batch of nanoEase M/Z Peptide BEH C18 packing material is chromatographically tested with acidic, basic, and neutral analytes, with the results constrained to narrow specification ranges to assure exceptional, consistent performance. Each column is individually evaluated, and an eCordTM Intelligent Chip is used to produce a Performance Chromatogram and a Certificate of Batch Analysis.
The nanoEase M/Z Peptide BEH C18 Column's pre-assembled ZenFit Connectors are made to fit into connection ports with a V-detail appearance. The V-detail connection port is a 10-32 threaded port for 1/16" O.D. tubing that is simple to use, reusable, fingertight, and can withstand pressures at 90°C. This makes it easier to construct crucial nano- and microflow UPLC fluid connections. Using the nanoEase M/Z Peptide BEH C18 Column, flow-path-induced variability in chromatographic results may be effectively eliminated.
You may also want to explore the Neutrals QC Reference Material. It contains one ampule with 10 μL/mL acetone, 0.25 mg/ mL naphthalene, and 0.4 mg/ mL acenaphthene in 50/50 acetonitrile/water, 2 mL. The Neutrals QC Reference Material can be used with any chromatographic system that has a UV detector. It is meant to be used as a benchmarking standard for systems. Only neutral chemicals are present in the mixture.
Across What pH Range Does The nanoEase M/Z Peptide BEH C18 Column Operate?
The nanoEase M/Z Peptide BEH C18 Column listed here is able to conduct analysis within the 1-10 pH range.
What Carbon Load Is Offered By The nanoEase M/Z Peptide BEH C18 Column?
This particular nanoEase M/Z Peptide BEH C18 Column carries a carbon load of 12%.