Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
10 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
10-32 for 1/16" male /360um open |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
185 |
System |
Nano/Micro |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
75 µm |
Length |
200 mm |
Carbon Load |
18 % |
UNSPSC |
41115711 |
Application |
Peptide |
Brand |
nanoEase M/Z |
Product Type |
Columns |
Units per Package |
1 pk |
nanoEase M/Z Peptide BEH C18 Column, 130Å, 1.7 µm, 75 µm X 200 mm, 1/pk
Waters’ family of nanoEase M/Z Columns are designed for optimal performance in nano-scale to micro-scale liquid chromatographic separations for biomarker discovery as well as protein and peptide identification and characterization. The nanoEase M/Z Peptide BEH C18 Column offers MS detection under UPLC conditions up to 15 kpsi (1,000 bar) operating pressures, as well as handling pressure fluctuations during injection well, allowing for superior longevity.
nanoEase M/Z Peptide BEH C18 Columns are equipped with a ZenFit connector. This easy-to-use, finger-tight, tool-free liquid connection is designed to ensure reproducible and leak-free connections between lab equipment. These fittings are compatible with Waters V-Detail ports, as well as fitting into Rheodyne/Vici compatible valves. ZenFit connectors are designed to improve the reproducibility of connections while minimizing dead volume in the flow path that can cause peak tailing and poor efficiency. This technology does not require user training or further special attention.
This column has a 1.7 mm particle size and a pore size of 130Å, with an inner diameter of 75 µm and a length of 200 mm. Operating limits include a pH minimum of 1 and a pH maximum of 10. Other qualities are available such as the nanoEase M/Z Peptide BEH C18 Column, 300Å, 1.7 µm, 75 µm X 200 mm, 1/pk. Each package contains one column, and each nanoEase M/Z Peptide BEH C18 Column is individually tested to ensure that it meets stringer quality controls. These measures ensure each column reaches its full potential for sensitivity, resolution, and reproducibility for biomarker discovery as well as for identifying and characterizing peptides and proteins.
What Mobile Phases and Buffers Should Be Used With nanoEase M/Z Peptide BEH C18 Columns?
In order to maintain and ensure the maximum lifetime and performance of your column, only MS-grade solvents should be used. Prior to use, filter all aqueous buffers through a 0.2 µm filter. For solvents containing suspended particulate materials, they may clog the inlet of the columns, leading to higher backpressure or distorted peak shape. Recommended buffers can be found in the Care and Use Manual provided with columns. Only prepare small quantities of mobile phase into fresh, clean bottles. Do not top-up mobile phases.