SKU: 186008792
nanoEase M/Z Peptide BEH C18 Column, 130Å, 1.7 µm, 75 µm X 100 mm, 1/pk

nanoEase M/Z Peptide BEH C18 Column | 186008792


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Product Description

nanoEase M/Z Peptide BEH C18 Column, 130Å, 1.7 µm, 75 µm X 100 mm columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. Like all M/Z columns they withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 130Å pore, C18, 1.7 µm particles. The small-pore (130Å) trifunctionally bonded BEH particle offers the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) to before being QC accepted as a Peptide separation column. The columns are equipped with the ZenFit connector, an easy to use, fingertight, tool free liquid connection. This ensures reproducible, leak free connections. The fittings will fit into Waters V-Detail ports as well as into Rheodyne/Vici compatible valves.

Suggested Uses: Bottom-up Proteomics, Nano-flow, Trap and Elute

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    10 pH

  • Maximum Pressure

    15000 psi (1034 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Endfitting Type

    10-32 for 1/16" male /360um open

  • Pore Size

    130 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    185

  • System

    Nano/Micro

  • Particle Technology

    BEH

  • USP Classification

    L1

  • Inner Diameter

    75 µm

  • Length

    100 mm

  • Carbon Load

    18 %

  • UNSPSC

    41115711

  • Application

    Peptide

  • Brand

    nanoEase M/Z

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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nanoEase M/Z Peptide BEH C18 Column, 130Å, 1.7 µm, 75 µm X 100 mm, 1/pk

Use the nanoEase M/Z Peptide BEH C18 Column for the best results in liquid chromatographic separation at the nano- to microscale. The lab equipment offers the best sensitivity, resolution, and repeatability for biomarker discovery and protein identification and characterization.

The nanoEase M/Z Peptide BEH C18 Column version presented below uses Waters' proprietary 130A pore, C18, 1.7 m particles. Making sure appropriate connections are made to prevent peak tailing and wasteful operation helps to achieve optimal performance.

To guarantee that you only receive the best lab equipment, each batch of the nanoEase M/Z Peptide BEH C18 Column is QC tested utilizing a gradient separation of a tryptic digest of cytochrome c.

The nanoEase M/Z Peptide BEH C18 packing materials were developed specifically for use with the ACQUITY UPLC System and are created in a cGMP, ISO 9001-certified facility using ultra-pure reagents. To achieve exceptional, repeatable performance, each batch of nanoEase M/Z Peptide BEH C18 packing material is chromatographically tested with acidic, basic, and neutral analytes. The results are constrained to narrow specification ranges. An eCordTM Intelligent Chip is used to give a Performance Chromatogram and a Certificate of Batch Analysis after each column is independently analyzed.

In order to give the same ease of use as more conventional columns, the nanoEase M/Z Peptide BEH C18 Column incorporates an ultra-low dispersion design. The nanoEase M/Z Peptide BEH C18 Column can resist high working pressures and handle pressure fluctuations during injection extraordinarily well, making it equivalent to other lab equipment with similar chemistries. This results in an increased lifetime.

The nanoEase M/Z Peptide BEH C18 Column's pre-assembled ZenFit Connectors are made to fit into connection ports with a V-detail appearance. The 10-32 threaded V-detail connection port is fingertight, reusable, and designed for 1/16" O.D. tubing. It can withstand high pressures at 90°C. Establishing crucial nano- and microflow UPLC fluid connections are made easier as a result. With the nanoEase M/Z Peptide BEH C18 Column, flow-path-induced variability in chromatographic results may be effectively eliminated.

You may also want to explore a similar product, the nanoEase M/Z HSS T3 Column, 100Å, 1.8 µm, 75 µm X 200 mm, 1/pk.

What Is The pH Range Of The nanoEase M/Z Peptide BEH C18 Column?

The nanoEase M/Z Peptide BEH C18 Column listed here offers a broad pH range of 1-10.

What Is The Maximum Pressure That The nanoEase M/Z Peptide BEH C18 Column Can Sustain?

The nanoEase M/Z Peptide BEH C18 Column can sustain a maximum of 15,000 psi (1034 Bar) pressure.