Waters PAH Columns optimized for HPLC analysis of Polyaromatic hydrocarbons (PAHs).

SKU: 186008760
XSelect Peptide HSS T3 Column, 100Å, 2.5 µm, 2.1 mm X 150 mm, 1K - 15K, 1/pk

XSelect Peptide HSS T3 Column | 186008760


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Product Description

XSelect Peptide HSS T3 Column, 100Å, 2.5 µm, 2.1 mm X 150 mm, 1K - 15K Columns are designed for separations where silica-based selectivities are desired or when increased retention of hydrophilic peptides are required. Each batch of HSS T3, 100Å material is QC tested with a gradient separation of a tryptic digest of cytochrome c (p/n: 186006371) and must pass stringent performance criteria before acceptance for use in these columns. 2.1mm ID columns are recommended for use with low dispersion (UPLC or UHPLC) systems, especially for LC-MS methods.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    2 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    45 C

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Bonding Technology

    T3

  • Silanol Activity

    Medium

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    15000

  • Particle Shape

    Spherical

  • Particle Size

    2.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    100 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    230

  • System

    UHPLC

  • Particle Technology

    HSS

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    150 mm

  • Carbon Load

    11 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    XSelect

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XSelect Peptide HSS T3 Column, 100Å, 2.5 µm, 2.1 mm X 150 mm, 1K - 15K, 1/pk

Peptide HSS T3 Columns are made for separations that call for greater retention of hydrophilic peptides or silica-based selectivities. The mechanical stability required to survive the high pressures present in UPLC separations is not present in high-pore volume HPLC particles. To meet this problem, the material scientists at Waters created silica particles with the ideal shape for excellent mechanical stability, resulting in long UPLC column lifetimes and high UPLC efficiencies at high pressures.

By choosing our tools, software, chemistries, and services, you have all the resources you require to complete any analysis successfully. For consistent, optimal performance of your Waters systems, only Waters Quality Parts have been evaluated and verified, making them compatible with your Waters instruments to the fullest extent. You can depend on reliable instrument performance, precise and repeatable results, and ongoing system compliance when you use Waters Quality Parts.

Waters has a wide variety of products to ensure you can fulfill all of your laboratory needs because we are aware that not every piece of equipment can be utilized for every sort of analysis. To shop for lab equipment, you can look through our catalog or visit our website. You can also contact one of our international customer service representatives through our website, who will help you with any questions or concerns you might have and propose the best equipment for your needs.

You may want to check out MassPREP ADH Digestion Standard; Yeast Alcohol Dehydrogenase (ADH, SwissProt P00330), which is used to make Waters MassPREPTM ADH, is digested using sequencing-grade trypsin. No standard protein, trypsin, or other hydrophilic substances are present in the finished protein digest. Every protein digestion standard batch is made in accordance with stringent quality control standards. The Certificate of Analysis report that is included contains the test results for each batch of the digestion standard. The performance evaluation of HPLC columns, high performance liquid chromatography, and mass spectrometry equipment used for the analysis of this class of substances are just a few of the uses for MassPREPTM Protein Digestion Standards.

What Techniques Are Advisable For Isolating And Preparing Analytes?

For the isolation and preparation of specific sample components, two commonly employed solid-phase extraction (SPE) techniques exist. When the concentrations of target chemicals are too low for precise quantification, the retention-cleanup-elution approach is typically utilized. This method allows for the concentration of diluted samples and the enrichment of trace chemicals. Alternatively, a pass-through cleanup method can be employed when the desired sample component is present in high concentrations. However, it is important to note that the pass-through cleanup approach does not result in sample enrichment.