Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
150000 |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
QC Tested |
Glycan |
Format |
Column |
Surface Area |
185 |
System |
UHPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
3 mm |
Length |
150 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Glycan |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Glycan BEH Amide Column, 130Å, 2.5 µm, 3 mm X 150 mm, 1K - 150K, 1/pk
The Waters XBridge Glycan BEH Amide Column is essential for retaining very polar substances such as carbohydrates and sugars. The lab equipment allows for the separation of polar analytes based on a range of characteristics such as polarity, structural moiety, and pKa. The XBridge Glycan BEH Amide Column provides a chemically stable, trifunctionally linked amide phase that is stable over a wide pH range.
The XBridge Glycan BEH Amide Column, which is based on Waters' innovative ethylene bridged hybrid (BEH) particle, uses a chemically stable, trifunctionally bound amide phase to provide exceptional stability and adaptability in HILIC separations laboratory equipment. Waters developed this capability as a novel solution to the problem of maintaining and separating very polar chemicals in HILIC stationary phases. Furthermore, the XBridge Glycan BEH Amide Column has the same selectivity as the ACQUITY UPLC BEH Amide columns, allowing scientists to smoothly transfer separations across HPLC and UPLC technology platforms.
Each batch of BEH Glycan material is quality control tested with the 2-AB labeled Waters Glycan Performance to ensure batch-to-batch consistency and highly identical separated glycan profiles. In addition to this, chromatographers can now select the most appropriate LC-based technology to address their individually released glycan analysis application needs and laboratory instrumentation to ensure extremely equivalent results. Waters BEH-based Glycan chemistry is available in three highly scalable particle sizes to meet the needs of UPLC (1.7 m) and HPLC (2.5 m XP and 3.5 m) applications. You can learn more about the variations of the lab equipment through our online store. The online store also helps you to match compatible items to get the best analysis experience as well as to purchase the lab equipment straight from us.
Waters recommends that you also explore and invest in the2-AB Glycan Performance Test Standard. The 2-AB Glycan Performance Test Standard is QC validated to contain the components needed to benchmark and assess ACQUITY UPLC BEH Glycan, 1.7 m Columns. It comes as a lyophilized powder in a Waters TruView vial. The total pmol in each vial is 228. This standard, like the QCRM product line, should be utilized on a regular basis for assessing column and system performance.
What Is The pH Range Offered By The XBridge Glycan BEH Amide Column?
This particular XBridge Glycan BEH Amide Column is able to work well within a broad operating pH range of 2-11.