SKU: 186007643
XBridge Protein BEH SEC Column, 450Å, 3.5 µm, 7.8 mm X 300 mm

XBridge Protein BEH SEC Column | 186007643

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Product Description

XBridge Protein BEH SEC, 450 Å Columns, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns.   They are best suited to separate proteins ranging from 100K – 1,500K Daltons.  Note: P/N 1763596 contains an XBridge Protein BEH SEC, 450 Å, 3.5um  7.8 x 300 Column with one vial of BEH450 SEC Protein Std (P/N 186006842)

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • Bonding Technology

    SEC

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    450 Å

  • QC Tested

    Protein

  • Format

    Column

  • System

    HPLC

  • Inner Diameter

    10 mm

  • Length

    300 mm

  • UNSPSC

    41115709

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk
Hazaradous Material This product contains hazardous material which requires special freight handling. Additional charges may apply.

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FAQs about XBridge Protein BEH SEC Column

What distinguishes the XBridge Protein BEH SEC Column from other SEC columns for protein analysis?
The XBridge Protein BEH SEC Column offers a large pore size of 450Å, which is particularly suitable for the analysis of high molecular weight proteins and protein complexes, providing superior resolution for these larger species.
How does the XBridge Protein BEH SEC Column contribute to the analysis of protein aggregates?
The XBridge Protein BEH SEC Column's design enables the effective separation of protein aggregates from monomeric forms, which is crucial in biotherapeutic development where aggregation can affect efficacy and immunogenicity.
Can the XBridge Protein BEH SEC Column be used in both aqueous and organic mobile phases?
The XBridge Protein BEH SEC Column is typically used with aqueous mobile phases for size exclusion chromatography, as organic solvents may disrupt the native state of proteins, which is critical for size-based separations.
Is the XBridge Protein BEH SEC Column suitable for use with multi-angle light scattering (MALS) detectors?
Yes, the XBridge Protein BEH SEC Column is compatible with MALS detectors, allowing for the determination of absolute molecular weights and sizes of proteins during SEC analysis.
What are the cleaning and storage recommendations for the XBridge Protein BEH SEC Column to ensure its performance and longevity?
To maintain the XBridge Protein BEH SEC Column, it is recommended to use appropriate cleaning protocols that include flushing with high-purity water and suitable buffers, and storing the column in a recommended storage solvent when not in use.

How Should The XBridge Protein BEH SEC Column Be Stored?

For overnight storage, continuously flush the column with the mobile phase at 10–20% of the maximum recommended flow rate. Store the column in the HPLC-grade water when it will be used within 24 hrs or in 20% methanol for long-term storage.