SKU: 186007639
XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 150 mm

XBridge Protein BEH SEC Column | 200Å | 3.5 µm | 7.8 mm X 150 mm | 1/pk | 186007639

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Product Description

XBridge Protein BEH SEC, 200 Å Columns, containing 3.5um particles are based on the same Waters Ethylene Bridged Hybrid (BEH)-based particle technology and diol-bonded surface coating as used in our successful line of HPLC-based SEC columns.   They are best suited to separate proteins ranging from 10,000 – 450,000 Daltons.  Note: P/N 176003595 contains an XBridge Protein BEH SEC, 200 Å, 3.5um  7.8 x 150  Column with one vial the BEH200 SEC Protein Std (P/N 186006518)

Specifications

  • Chemistry

    SEC

  • Separation Mode

    SEC

  • Particle Substrate

    Hybrid

  • Bonding Technology

    SEC

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    200 Å

  • QC Tested

    Protein

  • Format

    Column

  • System

    HPLC

  • Particle Technology

    BEH

  • Inner Diameter

    7.8 mm

  • Length

    150 mm

  • UNSPSC

    41115709

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

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XBridge Protein BEH SEC Column, 200Å, 3.5 µm, 7.8 mm X 150 mm

Compared to other silica-based size-exclusion chromatography (SEC) lab equipment, Waters XBridge Protein BEH SEC Columns deliver superior performance while allowing chromatographers to overcome a range of common challenges. These columns enable for easy development and transfer of SEC methods based on laboratory instrumentation, required protein component resolution, and sample throughput requirements. Made for use on HPLC instrumentation, these columns complement existing smaller particle size, UPLC-based SEC columns for use with proteins. XBridge BEH SEC Columns support reliable, high resolving SEC methods for the discovery, development, and quality assessment of protein-based biotherapeutics.

XBridge BEH SEC Columns are flow and pressure tolerant and have the ability to separate proteins between 10,000 and 450,000 Daltons, allowing for increased sample throughput on HPLC systems compared to traditional, silica-based SEC columns with particles below 5 µm.

Based on Waters’ Ethylene Bridged Hybrid (BEH) particle technology, the XBridge BEH SEC Column can be used in chromatography for a range of biological compounds, offering stability and performance attributes not found in many other traditional 100% silica-based particles. The combination of the BEH particle and an innovative diol bonding process allows for improved column stability, performance, and lifetime that is unprecedented. Lifetime can be further extended using the XBridge Protein BEH SEC Guard Column, 200Å, 3.5 µm, 7.8 mm X 30 mm.

All columns are synthesized in an ISO-certified manufacturing facility using high-quality raw materials. They are all thoroughly quality controlled and tested using a synthetic process, with each material tested against relevant proteins in order to ensure consistency across batches and offer confidence in all validated methods.

What Protein Standard Should I Use with XBridge Protein BEH SEC Columns?

Each XBridge Protein BEH SEC Column is shipped with a lyophilized vial of the appropriate BEH SEC Protein Standard Mix. These are the same protein standard formulation used by Waters manufacturing to ensure SEC Column reproducibility between batches. These same standards can be purchased through Waters, allowing chromatographers to use these same materials to benchmark new SEC columns or troubleshoot issues that may have arisen in a validated method. If repurchasing, be sure to choose the standard that matches the pore and particle size of your column.