ACQUITY UPLC M-Class Trap Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
Trap columns allow the scientist to perform complex 2D separations and to enrich and re-focus the sample before eluting to the nano-separation column for the final separation. The 2D Trap Columns are designed for 2D applications where the first dimension utilizes high pH. The unique combination of fritting technologies allows for the high pH compatibility. This allows the trap columns to withstand pressure changes during injection and thus provide superior longevity. This specific offering contains Waters Symmetry 100 Å pore, C18, 5 µm particles. Symmetry silica particles offer on average the highest retention for peptides and are thus most suitable for trapping applications.
The 2D V/M trap columns connect with a V-detail to the main valve and a smaller M-detail fitting to a T-piece or other suitable connection point in the LC system. Due to their design, 2D trap columns are suitable for bi-directional flow.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters(male)/Valco(male) |
Pore Size |
100 Å |
Format |
Trap Column |
Surface Area |
335 |
System |
Nano/Micro |
Particle Technology |
Silica |
USP Classification |
L1 |
Inner Diameter |
180 µm |
Length |
20 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Brand |
ACQUITY UPLC M-Class |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC M-Class Symmetry C18 Trap Column, 100Å, 5 µm, 180 µm x 20 mm, 2D, V/M, 1/pk
The packing materials produced in the ACQUITY UPLC M-Class Symmetry C18 Trap Column are produced in a cGMP, ISO 9001-certified facility using ultra-pure reagents and were created expressly for use with the ACQUITY UPLC System. To ensure good, repeatable performance, each batch of the packing material is chromatographically tested with acidic, basic, and neutral analytes, and the results are held to limited specification ranges. On the eCordTM Intelligent Chip, a Performance Chromatogram and Certificate of Batch Analysis are supplied after each column has undergone an individual test.
The ACQUITY UPLC M-Class Column, which is manufactured in compliance with exacting specifications, is fitted using a special frit technique. It can withstand high pressures because of these stringent requirements and the chemistry of the column. Additionally, the lab equipment can effectively handle pressure variations during injection, which increases longevity. Use our website to find more products that meet every challenge in your lab. The website also allows you to shop for lab equipment that is authentic, directly from us, and at the best available rates in the market.
Capillary-flow systems may necessitate more meticulous performance on a daily basis than ordinary flow chromatography does. It is crucial to make the right connections to reduce peak tailing and inefficient operation in order to obtain optimal performance.
You may also want to explore the MassPREP E. coli Digest Standard. The Waters Protein Expression System's MassPREP E. coli digestion standard (MPDS E. coli) is designed to offer a complex biological matrix for creating samples used to train new users. MPDS E. coli was created by using sequencing-grade trypsin to digest a pure E. coli cytosolic protein fraction. The MPDS mixture is purified and free of trypsin, undigested protein, salts, and other substances with a high hydrophilicity.
What Are The Upper And Lower pH Limits For The ACQUITY UPLC M-Class Symmetry C18 Trap Column?
For the ACQUITY UPLC M-Class Symmetry C18 Trap Column listed here, the lower limit for pH is 2, while the upper limit is 8.
Is There A Maximum Pressure For The ACQUITY UPLC M-Class Symmetry C18 Trap Column?
The ACQUITY UPLC M-Class Symmetry C18 Trap Column listed here can tolerate a maximum of 15,000 psi (1034 Bar) pressure.