ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation for biomarker discovery and protein and peptide identification and characterization. All M-Class columns are designed for 15 kpsi (1,000 bar) operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity.
This specific offering contains Waters patented BEH Technology 300 Å pore, C18, 1.7um particles. BEH technology offers the highest retentivity for basic compounds and provides excellent peak shape at elevated pH mobile phases and is suitable for separations up to 80°C. BEH is the universal column choice for a variety of compounds.
Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide Separation Column.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
10 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Valco(male)|360um Open |
Pore Size |
300 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
90 |
System |
Nano/Micro |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
150 µm |
Length |
100 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC M-Class |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC M-Class Peptide BEH C18 Column, 300Å, 1.7 µm, 150 µm X 100 mm, 1/pk
Make use of the ACQUITY UPLC M-Class Peptide BEH C18 Columns in your lab since they are made for the best performance in liquid chromatographic separation on a nano- to micro-scale. For the discovery of biomarkers and the identification and characterization of proteins, the lab equipment provides the highest levels of sensitivity, resolution, and repeatability. NanoACQUITY UPLC® Systems are compatible with many types of laboratory equipment.
The right connections must be made to reduce peak tailing and inefficient operation in order to achieve optimal performance. This is significant because, in contrast to conventional flow chromatography, capillary-flow systems may necessitate greater attention to detail for good day-to-day operation. To provide exceptional performance, the item on this list incorporates patented Waters BEH Technology 300Å, C18, 1.7 µm particles. Before being QC recognized as a Peptide Separation Column, each batch of the packing material used in the column's production is evaluated by utilizing a gradient separation of a tryptic digest of cytochrome C.
A unique frit method is used to frit the ACQUITY UPLC M-Class Peptide BEH C18 Column, which is produced in accordance with rigorous specifications. Due to these strict requirements and the chemistry of the lab equipment, it can endure high pressures. Additionally, it can manage pressure changes during injection quite well, which results in improved longevity.
Each ACQUITY UPLC M-Class Peptide BEH C18 Column is individually inspected to guarantee that it meets rigorous quality standards, ensuring that you only receive high-quality lab equipment. The column is produced in a closely watched and rigorously regulated atmosphere at a Waters facility that has ISO 9001 certification.
Add the MassPREP Peptide Mixture to your collection of lab equipment. A void volume marker and nine carefully chosen peptides with a wide variety of polarities and isoelectric points are included in the MassPREP peptide standard mixture. Under the reversed-phase HPLC conditions frequently used for peptide separations, all peaks are easily resolved. The MassPREP Peptide Standard Mixture is excellent for testing HPLC peptide separation systems and columns.
What Is The pH Range For The ACQUITY UPLC M-Class Peptide BEH C18 Column?
The Waters ACQUITY UPLC M-Class Peptide BEH C18 Column listed here is able to function well across the 1-10 pH range.
What Is The Maximum Pressure Recommended For The ACQUITY UPLC M-Class Peptide BEH C18 Column?
This ACQUITY UPLC M-Class Peptide BEH C18 Column has been designed to be able to withstand high pressures. The maximum recommended pressure for it is 15000 psi (1034 Bar).