ACQUITY UPLC M-Class Columns are designed for optimal performance for nano- to micro scale liquid chromatographic separation, offering the highest possible sensitivity, resolution and reproducibility for biomarker discovery and protein identification and characterization. As the 75µm to 180µm ID columns are fritted with a novel frit technology, they are able to withstand 15kpsi operating pressure and handle pressure fluctuations during injection extremely well and thus provide superior longevity. In order to achieve maximum performance, it is important to ensure that proper connections are made to minimize peak tailing and poor efficiency. This specific offering contains Waters patented BEH Technology 130A pore, C18, 1.7um particles. Each batch is specifically QC tested using a gradient separation of a tryptic digest of cytochrome c to before being QC accepted as a Peptide separation column.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
10 pH |
Maximum Pressure |
15000 psi (1034 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Valco(male)|360um Open |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
185 |
System |
Nano/Micro |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
150 µm |
Length |
100 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC M-Class |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC M-Class Peptide BEH C18 Column, 130Å, 1.7 µm, 150 µm X 100 mm, 1/pk
The ACQUITY UPLC M-Class Columns are designed for optimal sample recovery and enhanced chromatographic resolution by utilizing sub-2um particles efficiently. When combined with a mass spectrometer, the ACQUITY UPLC M-Class Columns provide the best sensitivity, resolution, and reproducibility for biomarker discovery and protein identification and characterization.
ACQUITY UPLC M-Class Peptide BEH C18 Columns are optimized for nano- to micro-scale liquid chromatographic separation, providing the best sensitivity, resolution, and repeatability for biomarker discovery, protein identification, and characterization.
Due to their innovative frit technology, the 75- to 180-µm ID columns are able to resist operating pressures of 15 kpsi, handle pressure changes during injection, and provide an outstanding lifetime. It is critical to ensure adequate connections are made to reduce peak tailing and poor efficiency in order to achieve optimal performance. Waters' patented BEH Technology 130A pore, C18, 1.7µm particles are used in this offering. Before being accepted as a Peptide separation column, each batch is QC tested utilizing a gradient separation of a tryptic digest of cytochrome c.
Waters ACQUITY UPLC M-Class Peptide BEH C18 Columns consistently provide excellent quality and the desired results for liquid chromatography. These columns, like all Waters equipment, are built in dedicated state-of-the-art Waters facilities. All Water manufacturing plants and facilities adhere to the most stringent cGMP and ISO 9001 standards. Each stage of the manufacturing process is meticulously checked and managed, ensuring that the equipment in your labs is of the greatest quality.
Visit our website to learn more about the ACQUITY UPLC M-Class Peptide BEH C18 Column variations or to find more goods that work with it; you may shop for lab equipment, refer to our catalog, and ensure your lab needs are addressed as needed. Our website also enables you to touch base with a member of our global support team if you need any assistance.
You may also be interested in the MassPREP Peptide Mixture, which contains a void volume marker and nine carefully selected peptides with a wide range of polarities and isoelectric points. Under reversed-phase HPLC settings commonly employed for peptide separations, all peaks are easily resolved. The MassPREP™ Peptide Standard Mixture is used for evaluating HPLC columns and peptide separation systems.
What Are The Operating Limitations Of The ACQUITY UPLC M-Class Peptide BEH C18 Column?
The ACQUITY UPLC M-Class Peptide BEH C18 Column can withstand operating pressures of up to 15000 psi (1034 Bar) and a pH range of 1 to 10, but working at severe pressures, pH, and/or temperatures will result in shorter column lifetimes.