Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
10 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Molecular Weight Range Max |
150000 |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
300 Å |
QC Tested |
Protein |
Format |
OBD Prep Column |
Surface Area |
90 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L26 |
Inner Diameter |
19 mm |
Length |
250 mm |
Carbon Load |
8 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Preparative Columns |
Units per Package |
1 pk |
XBridge Protein BEH C4 OBD Prep Column, 300Å, 5 µm, 19 mm X 250 mm, 1K - 30K, 1/pk
Waters Protein Separation Technology (PrST) columns have been quality-controlled and tuned to separate proteins by size, hydrophobicity, and isoelectric point. When comparing standard and C18 phases, the C4 ligand is less retentive, which means it will reduce protein carryover, improve protein recovery, and boost peak capacity.
The packing material in the XBridge Protein BEH C4 OBD Prep Column is designed to give excellent peak shape, high efficiency, and good recovery for biological macromolecules that are too big or hydrophobic to separate on columns with smaller holes or longer chain-linked phases. The base particle and bonding chemistry of the XBridge Protein BEH C4 OBD Prep Column have been chosen to ensure remarkable stability at both high and low pH, as well as high temperatures.
Like all products supplied by Waters as Waters-made, the XBridge Protein BEH C4 OBD Prep Column is created in a cGMP, ISO9002-certified plant utilizing ultra-pure reagents. A protein test mixture has also been used to qualify each batch of XBridge Protein BEH C4 OBD Prep Column packing material. The test findings are held to narrow specification ranges to ensure repeatable performance across the analytical column's lifespan. Every column is carefully tested to verify its efficiency, and each column comes with a Performance Test Chromatogram and a Certificate of Acceptance.
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You may also be interested in AutoPurification System Standard; The portfolio of Quality Control Reference Materials is a one-of-a-kind set of standards and mixtures. Before analyzing vital material, these items allow the user to analyze and benchmark their chromatography equipment. The products in the portfolio have all been specially formulated using Waters scientists' expertise. The AutoPurification Dye Standard contains 3 chemicals, including Thionin, Thioflavin, and Crystal Violet. Through fraction collection, this standard mix is utilized to confirm the benchmark performance of a Preparative/Purification System. This QCRM was created with the goal of allowing the user to double-check fraction collecting. This test is critical to run on the first use/installation of the system, as well as every time the system has not been used or has undergone any changes.
What Are The Operating Limitations Of The XBridge Protein BEH C4 OBD Prep Column?
The XBridge Protein BEH C4 OBD Prep Column can be easily operated within the 1 to 10 pH range, along with a maximum pressure of 6,000 psi (or 415 Bar).