Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
11 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
15000 |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
Prep Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
CSH |
USP Classification |
L1 |
Inner Diameter |
4.6 mm |
Length |
250 mm |
Carbon Load |
15 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
XSelect |
Product Type |
Preparative Columns |
Units per Package |
1 pk |
XSelect Peptide CSH C18 Prep Column, 130Å, 5 µm, 4.6 mm X 250 mm, 1K - 15K, 1/pk
For peptides utilizing either FA- or TFA-containing mobile phases, Waters XSelect CSH130 C18 5 µm particles have a low and precisely controlled concentration of positive charges, producing comparatively excellent peak shape. Ideal for LC or LC-MS applications, a CSH130 C18 column's performance shows little dependence on strong ion pairing agents. General-purpose C18 columns with a higher level of selectivity than other C18 sorbents are called XSelect CSH C18 columns. Charged Surface Hybrid (CSH) technique improves peak form and loading capacity for fundamental chemicals.
The Waters XSelect CSH C18 XP Column offers unmatched selectivity and reproducibility for your chromatographic analysis. The objective of the lab apparatus was to separate components of closely eluting samples utilizing a range of analyte selectivity. The column can be used as a general-purpose column that provides alternative selectivity as compared to other commercially available C18 sorbents.
You can address column-related problems such as asymmetry in peak form, insufficient loading for fundamental chemicals, column bleed, and sluggish mobile-phase re-equilibration with the use of the column's built-in Charged Surface Hybrid (CSH) technology. You can accomplish greater resolution separations with the XSelect CSH C18 XP Column, which are required for isolating and quantifying challenging samples. This is possible because the special phases of the analytical column combine efficiency and selectivity to enhance the resolution of extremely complex samples.
The lab equipment is fully compatible with all HPLC, UPLC, and UHPLC technologies, which will save you time and effort when developing a chromatographic test. You might also find it interesting that the analytical column's strong batch-to-batch repeatability enables you to quickly establish reliable processes.
Depending on your requirements, you can shop for lab equipment by exploring our website or our catalog. On our website, you may also browse the full selection of Waters services and goods, including extra lab supplies that work with the XSelect CSH C18 Column.
You should also review LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; All Waters LCMS Certified vials are produced following tightly controlled manufacturing processes and handling procedures. They are tested with mass spectrometers and come packaged with a certificate of analysis showing the reference and vial scan for the manufacturing lot.
Could Water Be Used As A Mobile Phase?
Pure water at a high temperature can be used as a mobile phase in liquid chromatography due to the change in dielectric constant. Water has an organic solvent behavior, which causes its dielectric constant to drop from 85 at 25°C to 35 at 200°C.