Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
15000 |
Particle Shape |
Spherical |
Particle Size |
10 µm |
Pore Size |
300 Å |
QC Tested |
Peptide |
Format |
Prep Guard Cartridge |
Surface Area |
90 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
30 mm |
Length |
10 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
XBridge |
Product Type |
Guards |
Units per Package |
1 pk |
XBridge Peptide BEH C18 Prep Guard Cartridge, 300Å, 10 µm, 30 mm X 10 mm, 1K - 15K, 1/pk
The XBridge Peptide BEH C18 30x10mm Prep Guard Cartridge, when used in conjunction with the 30x10mm Prep Guard Holder (part number 186006912), is intended to protect HPLC and SFC 30 mm ID Prep OBDTM columns. Placing this guard assembly ahead of the prep column will increase column lifetime and lower purification costs.
Between the injector and main column, we recommend using a Waters guard assembly with matching chemistry and particle size. It is critical to protect the main column with a high-performance matching guard column while not compromising or changing the analytical resolution. Guard assemblies must be replaced at predetermined intervals based on sample contamination. When the system back pressure steadily rises above a preset pressure limit, it is usually a sign that the guard column needs to be replaced. The sudden appearance of split peaks is also an indication that the guard assembly needs to be replaced.
For acidic and basic mobile phases, the XBridge™ packing materials were designed to provide excellent peak shape, high efficiency, and excellent stability. The XBridge™ packing materials are produced in a cGMP, ISO 9001:2000 certified facility using ultra-pure reagents. Each batch of XBridge™ material is chromatographically tested with acidic, basic, and neutral analytes, and the results are held to tight specification ranges to ensure excellent, repeatable performance. Each step of the manufacturing process is meticulously monitored and controlled, ensuring that the equipment in your labs is of the highest quality.
To ensure that you have quality products for your analytical needs, you can shop for lab equipment through the Waters online store on our website. You may also want to check out AutoPurification System Standard; The Quality Control Reference Materials portfolio is a one-of-a-kind collection of standards and mixtures. Prior to performing critical material analysis, the user can evaluate and benchmark their chromatography system using these products. The portfolio's products have all been specially formulated based on Waters scientists' expertise. The AutoPurification Dye Standard contains three compounds: thionin, thioflavin, and crystal violet. This standard mix is used to confirm the benchmark performance of a Preparative/Purification System via fraction collection. This test is extremely useful to run when the system is first used/installed, as well as whenever it has not been used or has undergone any changes.
What Role Does High Resolution Play in Chromatography?
Peak width and retention time, both inherent properties of the chromatographic process, determine resolution; resolution measurements can be used to optimize separations. The better and more accurately the results can be analyzed, the higher the resolution obtained.