ACQUITY UPLC Protein BEH SEC Column, 450Å, 2.5 µm, 4.6 mm X 150 mm, 100K - 1M, 1/pk

Based on Waters’ successful ethylene bridged hybrid (BEH) particle, the ACQUITY UPLC Protein BEH SEC Column is the first commercially available sub 2 µm column designed specifically for SEC chemistries. The columns have been designed and optimized for the analysis of proteins and their aggregates with molecular weights ranging from 100,000 to 1,500,000 Daltons. This allows for accurate aggregations at up to ten times the speed of traditional HPLC-based SEC methods.

The excellent performance of these columns is possible due to the BEH Diol coated particle technology, which allows for rapid separation and accurate quantitation of protein and peptide monomers from their respective aggregates. When chromatographers choose lab equipment based on this technology, they are able to feel confident in their measurements of aggregation species. Additionally, they can use less eluent than other SEC methods require and reduce the requirement for high salt concentration mobile phases. Many chromatographers note a decrease in non-desired, ionic secondary interactions between protein/peptide and SEC particle surfaces.

All ACQUITY UPLC Protein BEH SEC Columns are quality tested with relevant proteins and peptides in order to maintain unmatched consistency and reproducibility. Manufacturing is done in a cGMP, ISO 9001 certified plant and uses ultra-pure reagents, with all results held to narrow specification ranges that ensure high quality and reproducibility. Every batch of packing material is tested and qualified using BEH450 SEC Protein Standard Mix. Each column has been individually tested and includes a Performance Test Chromatogram and a Certification of Analysis.

Columns are sold individually and come one to a package.