Chemistry |
PFP/Fluoro-Phenyl |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Bonding Technology |
PFP |
Silanol Activity |
High |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
230 |
System |
UHPLC, UPLC |
Particle Technology |
HSS |
USP Classification |
L43 |
Inner Diameter |
2.1 mm |
Length |
100 mm |
Carbon Load |
7 % |
eCord |
Yes |
UNSPSC |
41115709 |
Brand |
XSelect |
Product Type |
Columns |
Units per Package |
1 pk |
XSelect HSS PFP Column, 100Å, 2.5 µm, 2.1 mm X 100 mm, 1/pk
The XSelect HSS PFP Column is one of the most dependable, reproducible, and scalable silica-based PFP LC columns on the market. The unique HSS substrate and PFP ligand combination provides true PFP performance without sacrificing peak shape or retention time variation. The XSelect HSS PFP Column employs High Strength Silica (HSS) technology. This method was designed with chromatography in mind, with benefits such as improved polar molecule retention and varied selectivity. The HSS particle has the mechanical strength to withstand UPLC pressures in addition to its analytical capabilities.
The XSelect HSS PFP column, a low coverage, non-end-capped pentafluorophenyl phase, offers superior separation of chemically identical compounds as well as alternate selectivity to standard alkyl chain columns due to a complex interplay of retention processes. The incorporation of 2.5 µm eXtended Performance [XP] columns in ultra-low dispersion hardware, which delivers higher separation performance, robustness, and throughput for HPLC testing, enables a smooth transition route to UPLC adoption.
The XSelect HSS PFP Column offers a unique phase for base and planar aromatic compound selectivity. When increased compound retention is required, particularly for polar basic analytes whose retention is difficult with C18 stationary phases, rely on the XSelect HSS PFP Column. Because PFP phases are electron acceptors, these ligands will interact with Lewis base-containing molecules pi-pi. The stiff aromatic ring provides increased selectivity based on analyte shape, dipole moment, and hydrogen bonding interactions.
To aid in the development of techniques, the XSelect HSS PFP Column is offered in a variety of particle sizes and column shapes. You can shop for lab equipment on our website and study the whole Waters product catalog.
You may also be interested in XSelect HSS PFP XP VanGuard Cartridge, 100 Å, 2.5 µm, 2.1 mm X 5 mm, 3/pk; XSelect HSS PFP XP VanGuard Cartridge, 100, 2.5 m, 2.1 mm X 5 mm, 3/pk; XSelect HSS PFP VanGuard Cartridges are used to improve the lifetime and performance of analytical columns by eliminating particle pollution from the mobile phase stream.
How Is Enrichment in Low Concentration Sample Components Achieved?
Several techniques can be done to enrich sample components that are present in low amounts. You can change the gradient stages to selectively elute analytes. Another effective strategy is to use "large" sample volumes in an adsorption-promoting solvent. Use a "small" collection volume in a desorption-friendly solvent. Rather of the chemistry present in the analytical column, use sorbent chemistry appropriate to the analyte. To avoid the need for additional sample preparation, it is critical to carefully determine the chemistry of the solid-phase extraction column.