SKU: 186006099
XBridge BEH Amide Column, 130Å, 2.5 µm, 4.6 mm X 100 mm, 1/pk

XBridge BEH Amide Column | 186006099


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Product Description

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

Specifications

  • Chemistry

    Amide

  • Separation Mode

    Hydrophilic Interaction (HILIC)

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    11 pH

  • Maximum Pressure

    10000 psi (690 Bar)

  • Endcapped

    No

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    2.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • Format

    Column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L68

  • Inner Diameter

    4.6 mm

  • Length

    100 mm

  • Carbon Load

    12 %

  • UNSPSC

    41115709

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XBridge BEH Amide Column, 130Å, 2.5 µm, 4.6 mm X 100 mm, 1/pk

The XBridge BEH Amide XP Column contains a tri-functionally connected amide phase that is pH stable from 2 to 11, allowing it to separate polar analytes with a wide range of polarity, structural moiety, and pKa. Your system will retain extremely polar compounds like carbohydrates and sugars when you use the XBridge BEH Amide XP Column. The BEH particle technology in the XBridge BEH Amide XP Column improves hydrolytic stability and particle strength, preventing any LC/MS leakage in the system.

The XBridge BEH Amide XP Column has a particle size of 2.5 m and is created within packaging utilizing ultra-low dispersion hardware. It is based on the eXtended Performance [XP] column technology, which allows for improved separation performance, robustness, and throughput for HPLC investigations while also facilitating the transfer to UPLC adoption.

XBridge XP Columns cover a comprehensive variety of options when it comes to HPLC, UHPLC, and UPLC systems. The XBridge BEH Amide XP Column is highly customizable, allowing you to easily develop robust methods with a wide range of mobile phases and pH ranges. This lab equipment will assist in developing samples with remarkable chemical stability at high pH and temperatures, even when the bulk loading of basic chemicals is increased.

To review additional collections of lab equipment from Waters, you may review our catalog or browse through our website, from where you can shop for lab equipment to fulfill all your laboratory requirements.

Compatible with the XBridge BEH Amide Column, you should check out the XBridge BEH Amide XP VanGuard Cartridge, 130Å, 2.5 µm, 3.9 mm X 5 mm, 3/pk; XBridge BEH Amide VanGuard Cartridges are used to extend analytical column lifetime and performance by removing particulate contamination from the mobile phase stream. This cartridge is optimized to protect all 4.6 mm I.D. XBridge BEH Amide analytical columns containing 2.5 µm sorbent particles.

What Is BEH's Importance In Chromatography?

One of the primary enablers underlying UPLC technology is the BEH (Ethylene Bridged Hybrid) particle, which comes in a variety of pore sizes and bonded phases, allowing for both reversed-phase and hydrophilic contact chromatography. The approach may be used to evaluate a variety of biopharmaceutical compounds, including tiny and big molecules. Because hybrid particle technology is chemically stable, it can be used in a larger pH range [pH 1-12]. As a result, it will be possible to design a diverse and reliable separation technology for method development. BEH particle technology comes in a variety of HPLC particle sizes [2.5, 3.5, 5, and 10 m], allowing for a seamless transition from HPLC to UPLC.