SKU: 186006098
XBridge BEH Amide Column, 130Å, 2.5 µm, 4.6 mm X 75 mm, 1/pk

XBridge BEH Amide Column | 186006098


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Product Description

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

Specifications

  • Chemistry

    Amide

  • Separation Mode

    Hydrophilic Interaction (HILIC)

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    11 pH

  • Maximum Pressure

    10000 psi (690 Bar)

  • Endcapped

    No

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    2.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • Format

    Column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L68

  • Inner Diameter

    4.6 mm

  • Length

    75 mm

  • Carbon Load

    12 %

  • UNSPSC

    41115709

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XBridge BEH Amide Column, 130Å, 2.5 µm, 4.6 mm X 75 mm, 1/pk

Extremely polar compounds, such as carbohydrates and sugars, are retained by XBridge BEH Amide columns. XBridge Amide columns provide a chemically stable, tri-functionally bonded amide phase that is pH 2 to 11 stable, allowing for the separation of polar analytes with a wide range of polarity, structural moiety, and pKa. XBridge BEH XP [eXtended Performance] Columns provide rugged and repeatable performance for all HPLC and UHPLC separation conditions, maximizing efficiency and retention. 2.5 µm eXtended Performance [XP] columns with ultra-low dispersion hardware provide exceptional separation performance, robustness, and throughput for HPLC assays while enabling a smooth transition path toward UPLC adoption.

XBridge XP Columns offer a wide range of options when it comes to HPLC, UHPLC, and UPLC systems. The XBridge BEH Amide XP Column is highly customizable, allowing you to quickly design robust procedures using a wide range of mobile phases. Even when the mass loading of basic chemicals is increased, this lab equipment will enable you to produce samples with exceptional chemical stability at high pH and temperatures.

To view additional Waters lab equipment collections, please consult our catalog or visit our website, where you can shop for lab equipment to meet all of your laboratory requirements.

Compatible with the XBridge BEH Amide Column, you may also be interested in the XBridge BEH Amide Column, you may also be interested in the XBridge BEH Amide XP VanGuard Cartridge, 130Å, 2.5 µm, 3.9 mm X 5 mm, 3/pk; XBridge BEH Amide VanGuard Cartridges are used to extend the lifetime and performance of analytical columns by removing particulate contamination from the mobile phase stream. This cartridge has been designed to protect all 4.6 mm I.D. XBridge BEH Amide analytical columns with 2.5 µm sorbent particles.

What Role Does BEH Play in Chromatography?

One of the main enablers underlying UPLC technology is the BEH (Ethylene Bridged Hybrid) particle, which is available in a variety of pore sizes and bonded phases, allowing for reversed-phase and hydrophilic contact chromatography. The method is applicable to a wide range of bio-pharmaceutical substances, including small and large molecules. Because hybrid particle technology is chemically stable, it can be used in a wider pH range [pH 1-12]. As a result, method development can benefit from a diverse and dependable separation technology. BEH particle technology is available in a range of HPLC particle sizes [2.5, 3.5, 5, and 10 µm], enabling a seamless transition from HPLC to UPLC platforms.