Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
10000 psi (690 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
4.6 mm |
Length |
75 mm |
Carbon Load |
12 % |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge BEH Amide Column, 130Å, 2.5 µm, 4.6 mm X 75 mm, 1/pk
Extremely polar compounds, such as carbohydrates and sugars, are retained by XBridge BEH Amide columns. XBridge Amide columns provide a chemically stable, tri-functionally bonded amide phase that is pH 2 to 11 stable, allowing for the separation of polar analytes with a wide range of polarity, structural moiety, and pKa. XBridge BEH XP [eXtended Performance] Columns provide rugged and repeatable performance for all HPLC and UHPLC separation conditions, maximizing efficiency and retention. 2.5 µm eXtended Performance [XP] columns with ultra-low dispersion hardware provide exceptional separation performance, robustness, and throughput for HPLC assays while enabling a smooth transition path toward UPLC adoption.
XBridge XP Columns offer a wide range of options when it comes to HPLC, UHPLC, and UPLC systems. The XBridge BEH Amide XP Column is highly customizable, allowing you to quickly design robust procedures using a wide range of mobile phases. Even when the mass loading of basic chemicals is increased, this lab equipment will enable you to produce samples with exceptional chemical stability at high pH and temperatures.
To view additional Waters lab equipment collections, please consult our catalog or visit our website, where you can shop for lab equipment to meet all of your laboratory requirements.
Compatible with the XBridge BEH Amide Column, you may also be interested in the XBridge BEH Amide Column, you may also be interested in the XBridge BEH Amide XP VanGuard Cartridge, 130Å, 2.5 µm, 3.9 mm X 5 mm, 3/pk; XBridge BEH Amide VanGuard Cartridges are used to extend the lifetime and performance of analytical columns by removing particulate contamination from the mobile phase stream. This cartridge has been designed to protect all 4.6 mm I.D. XBridge BEH Amide analytical columns with 2.5 µm sorbent particles.
What Role Does BEH Play in Chromatography?
One of the main enablers underlying UPLC technology is the BEH (Ethylene Bridged Hybrid) particle, which is available in a variety of pore sizes and bonded phases, allowing for reversed-phase and hydrophilic contact chromatography. The method is applicable to a wide range of bio-pharmaceutical substances, including small and large molecules. Because hybrid particle technology is chemically stable, it can be used in a wider pH range [pH 1-12]. As a result, method development can benefit from a diverse and dependable separation technology. BEH particle technology is available in a range of HPLC particle sizes [2.5, 3.5, 5, and 10 µm], enabling a seamless transition from HPLC to UPLC platforms.