SKU: 186006095
XBridge BEH Amide Column, 130Å, 2.5 µm, 3 mm X 100 mm, 1/pk

XBridge BEH Amide Column | 186006095


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Product Description

XBridge BEH Amide columns retain extremely polar compounds, including carbohydrates and sugars. XBridge Amide columns offer you a chemically stable, trifunctionally bonded amide phase that is stable from pH 2 to 11 to enable the separation of polar analytes spanning a wide range of polarity, structural moiety and pKa. Packed in ultra-low dispersion hardware, 2.5 µm eXtended Performance [XP] columns enable exceptional separation performance, robustness and throughput for HPLC assays while enabling a seamless transition path toward UPLC adoption.

Specifications

  • Chemistry

    Amide

  • Separation Mode

    Hydrophilic Interaction (HILIC)

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    11 pH

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    No

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    2.5 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    130 Å

  • Format

    Column

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L68

  • Inner Diameter

    3 mm

  • Length

    100 mm

  • Carbon Load

    12 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XBridge BEH Amide Column, 130Å, 2.5 µm, 3 mm X 100 mm, 1/pk

The XBridge BEH Amide Column presents a high-performance solution for reversed-phase liquid chromatography (LC) of amide-containing compounds, featuring a 130Å, 2.5µm sorbent bed bonded to a hybrid silica substrate for exceptional retention of polar and non-polar compounds without compromising target analytes.

Compatible with all major liquid chromatography systems, the XBridge BEH Amide Column caters to diverse applications, including amide mapping, identification, and quantitation of amide-containing compounds commonly found in proteins and peptides, enabling precise separation and quantification of complex mixtures.

The lab equipment is available in a single-use format for convenient and cost-effective operation, eliminating the need for regeneration and cleaning, as well as simplifying workflows. It is also available in other sizes and packs of three, like with the XBridge BEH Amide Column, 130Å, 2.5 µm, 3 mm X 100 mm, 3/pk.

With its 130Å, 2.5µm sorbent bed, the XBridge BEH Amide Column ensures superior retention of polar and non-polar compounds, complemented by a bonded hybrid silica substrate for enhanced durability and performance, making it a reliable choice for amide-focused LC analyses.

Moreover, the column's incorporation of polar groups further enhances the retention of basic compounds, contributing to its high selectivity and providing a powerful tool for the challenging separation of amide-containing compounds that may be difficult to isolate using other methods. The XBridge BEH Amide Column facilitates optimal LC system performance, extending the system's lifetime while simultaneously enhancing analytical output, and generating reliable, accurate, and reproducible results for a diverse range of applications, including amide mapping, identification, and quantitation.

This user-friendly column ensures easy installation and use, simplifying your laboratory protocols. With its single-use format, the XBridge BEH Amide Column streamlines your workflows, maximizing productivity and delivering cost-effective, high-performance LC analyses of amide-containing compounds, ultimately advancing your research and analytical capabilities.

What is the nominal pore size and particle size of the column?

The column has a nominal pore size of 130Å and a particle size of 2.5 µm.

What are the dimensions of the column?

The column has a length of 100 mm and a diameter of 3 mm.