Chemistry |
HILIC |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
9 pH |
Maximum Pressure |
10000 psi (690 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
2.5 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L3 |
Inner Diameter |
4.6 mm |
Length |
75 mm |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge BEH HILIC Column, 130Å, 2.5 µm, 4.6 mm X 75 mm, 1/pk
Use the unbonded BEH substrates in the Waters XBridge BEH HILIC columns to your advantage so you can keep track of and separate extremely polar chemicals that conventional reversed-phase chromatography struggles to hold on to. Because the BEH particle is more durable than silica-based HILIC phases, its chemical stability results in lengthy column lifetimes. Under HPLC HILIC conditions, the special lab equipment is tested, optimized, and used to produce effective, repeatable separations.
With the use of the XBridge BEH HILIC Column, polar analytes can easily achieve increased retention. Partitioning, ion exchange, and hydrogen bonding are all used in conjunction with complicated retention mechanisms made possible by the existence of the HILIC technology in the column. Because retention in this column occurs with a polar stationary phase mixed with mobile phases composed of acetonitrile concentrations of more than 80%, the XBridge Column also offers greater retention than the reversed-phase. In addition to this, the analytical column provides additional selectivity over reversed-phase columns. The XBridge BEH HILIC Column has also been used to observe a reversal in elution in addition to improved retentivity. When creating orthogonal separations for polar analytes, this is especially helpful.
There are other versions of the XBridge BEH HILIC Column as well. Use our catalog or the technology guides on our website to learn more about the chemistry of the column and shop for lab equipment directly from our website.
In contrast to conventional reversed-phase columns, you can use the XBridge BEH HILIC Column to benefit from alternate selectivity. Additionally, compared to HILIC columns made of silica, the lab equipment can offer benefits in peak form, retention, and column lifetime. The lab equipment sets a new benchmark for HILIC separations due to the durable and reliable BEH Technology on which the XBridge BEH HILIC Column is built. This is made feasible by the distinctive characteristics of Hydrophilic Interaction Chromatography and the analytical column (HILIC).
Protect your column from unwanted contaminants using the XBridge BEH HILIC XP VanGuard Cartridge, 130Å, 2.5 µm, 3.9 mm X 5 mm, 3/pk.
What Is The pH Range For The XBridge BEH HILIC Column?
The Waters XBridge BEH HILIC Column listed here is able to work well within the 1-9 pH range.
What Is The Maximum Pressure That The XBridge BEH HILIC Column Can Tolerate?
This particular XBridge BEH HILIC Column is able to tolerate a maximum of 10,000 psi (690 Bar) pressure.