ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 75 mm, 1K - 30K, 1/pk

Based on the chemically stable, trifunctionally bonded amide phase of the BEH particle, ACQUITY UPLC BEH Amide Columns enable a new dimension in stability and versatility. Waters’ focus on innovative lab equipment and stationary phases for HILIC (Hydrophilic Interaction Chromatography) has allowed this column to overcome the challenge of retaining and separating extremely polar compounds through use of the novel ethylene bridged hybrid particle.

ACQUITY UPLC BEH Amide Columns enable exceptional retention of polar analytes spanning a wide range in polarity, structural moiety, and pKa through the use of a wide range of mobile phase pH, from 2 to 12. Along with the enhanced retention of polar analytes and metabolites, the column offers mass spectrometry response, direct compatibility with sample preparation eluates (PPT, LLE, and SPE), and orthogonal selectivity when compared to other reversed-phase materials.

Analysis of carbohydrates, including monosaccharides, disaccharides, oligosaccharides, and polysaccharides, is improved through ACQUITY UPLC BEH Amide Columns’ use of BEH particles. This technology offers increased chemical stability that enables the use of both high pH and high temperature to collapse, reducing sugar anomers, shortening the analysis time, and improving MS detection. Assay robustness is also improved through the exceptional column lifetime provided by the BEH particle. Lifetime can be further improved through the use of ACQUITY UPLC BEH Amide VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk.

Unlike amine-based columns commonly used in carbohydrate analysis, the ACQUITY UPLC BEH Amide column is not vulnerable to Schiff-based formation, allowing for improved quantitation accuracy.