Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
30000 |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
300 Å |
QC Tested |
Peptide |
Format |
Column |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
1 mm |
Length |
150 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC Peptide BEH C18 Column, 300Å, 1.7 µm, 1 mm X 150 mm, 1K - 30K, 1/pk
The Waters ACQUITY UPLC Peptide BEH C18 Columns are unique as they are applicable for wide pore substances (300 Å) and are tri-functionally bonded. These columns offer a wide usable pH range (1 - 12) and exceptional low pH stability. They are critical pieces of lab equipment for any scientist as they offer ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.
Experience improved chromatography of peptides with the ACQUITY UPLC Peptide BEH C18 Column. It will assist you in achieving narrow, symmetrical peaks for best resolution, successful separations of a wide range of peptides, including large and small, acidic and basic, and hydrophobic and hydrophilic. You can be certain of a good peak shape and retention in formic acid and trifluoroacetic acid (TFA) for optimal chromatography and detection.
The ACQUITY UPLC Peptide columns are built on Waters Peptide Separation Technology (PST) that utilizes the BEH Technology particles; these columns meet the demanding specifications for the BEH Technology particles and are specifically QC certified with a peptide map. This ensures the results obtained will provide stability of well-developed peptide separations and predictable behavior with many different samples used in the proteomics, protein characterization, and peptide synthesis. The surface chemistry gives sharp symmetrical peaks over a wide range of peptide properties and application requirements.
Designed specially to be compatible with ACQUITY UPLC and ACQUITY PREMIER systems, the ACQUITY UPLC Peptide BEH C18 Columns are manufactured in a cGMP, ISO 9002 certified plant using ultra-pure reagents. Each batch is tested chromatographically with acidic, basic, and neutral analytes and has been qualified for peptide mapping. To assure you get exceptional lab equipment that provides reliable analysis and reproducible performance, the results are held to narrow specification ranges so that you get reproducibility and consistency, batch-over-batch and column-over-column.
Find various products that are compatible with the ACQUITY UPLC Peptide BEH C18 Column, including guards, vials, elution plates from our website. The website allows you to shop for lab equipment and connect with our staff with any questions you may have.
We also recommend checking out ACQUITY UPLC Peptide BEH C18 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm, 1K - 30K, 3/pk.
Do I Need To Use An Auto-Sampler?
Autosamplers and robotic workstations provide reproducible injections and allow automation of the chromatographic separation. Scientists need to keep in mind that along with their advantages, they will add a significant cost to your system. As an example, many worldwide university laboratories prefer to substitute graduate students to do the job.