ACQUITY UPLC Protein BEH C4 Column, 300 Å, 1.7 µm, 1 mm x 100 mm, 1/pk

The ACQUITY UPLC Protein BEH C4, 300 Å, 1.7 µm, 1 mm x 100 mm column is QC tested and optimized to separate proteins based on size, hydrophobicity, and isoelectric point. If you compare it to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.

When it comes to biological macromolecules that are too big or too hydrophobic to separate on columns with smaller pores or longer chain-linked phases, the ACQUITY Premier Protein BEH C4, 300 Å, 1.7 µm packing material excels. The lab equipment also offers high efficiency and good recovery. In addition to this, at both high and low pH levels as well as at high temperatures, the base particle and bonding chemistry are selected to offer remarkable stability.

The packaging materials for the ACQUITY UPLC Protein BEH C4 are produced in a cGMP, ISO9002 certified facility using ultra-pure reagents. To ensure reproducible performance, each batch of Protein Separation Technology column material has been validated with a protein test mixture, and the results are held to limited specification ranges. Every lab equipment is individually examined for effectiveness, and each column is accompanied by a Performance Test Chromatogram and a Certification of Acceptance.

The foundation of the ACQUITY UPLC Protein BEH C4 Columns is the 1.7 µm particles that define UltraPerformance LCTM Technology. Protein separations benefit from increased resolution, sensitivity, and speed as a result of the small particle size packing's reduction of dispersion and band broadening. It is general knowledge that with such tiny particles, extremely high system back pressures will be seen. Proteins, on the other hand, are big molecules that diffuse gradually; hence the ideal linear velocity is substantially lower for proteins than it is for small molecules. System pressure is low because the flow rate is low. Although the lab equipment does not require the high-pressure capability of an ACQUITY System, the low system volume and low detector dispersion of the optimized ACQUITY System are necessary for this column to function to its full potential.

The MaxPeakTM High Performance Surfaces found on the ACQUITY Premier Protein BEH C4 Columns are a cutting-edge innovation created to improve analyte recovery, sensitivity, and reproducibility by reducing analyte/surface interactions that can result in sample losses.

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