ACQUITY UPLC Oligonucleotide BEH C18 Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 30K, 1/pk
Depend on the 1.7 µm ACQUITY UPLC Oligonucleotide BEH C18 Column to deliver exceptional peak shape, sample resolution, and extended column life. The lab equipment is perfect for the characterization of oligonucleotides by ion-pair, reversed-phase chromatography. It has been designed for use with an ACQUITY UPLC System and is easily able to resolve large oligonucleotide sequences by leveraging the resolving power of sub-3 µm, BEH Technology particles.
The ACQUITY UPLC Oligonucleotide BEH C18 Column uses the Waters Oligonucleotide Separation Technology (OST) that is used to separate detritylated synthetic oligonucleotides based on ion-pair, reversed-phase chromatography. As each column is QC tested using Waters MassPrep OST standards, you can be certain of the repeatable performance for your most demanding oligonucleotide assays.
Meeting the demands of the UPLC analysis of synthetic oligonucleotides has been simplified with the introduction of the ACQUITY UPLC Oligonucleotide BEH C18 Column. The analytical column was specially designed in response to in-depth studies of existing technologies of this application area. This flexible separation chemistry technology was developed by Waters to ensure that researchers could make groundbreaking discoveries that lead to drug therapies or diagnostic reagents.
The ACQUITY UPLC Oligonucleotide BEH C18 Columns rely on the separation method based on highly efficient ion-pairing reversed-phase (IR-RP) chromatography of the ‘tritily-off’ synthetic oligonucleotide species, where the oligonucleotide is detritylated at the last step of the synthesis.
In addition to the features mentioned, the ACQUITY UPLC Oligonucleotide BEH C18 Column also provides a new standard of synthetic oligonucleotide purification by providing the highest level of purification of the product. This is achieved without compromising product recovery when compared with gel electrophoresis, desalting, cartridges, or ion-exchange chromatography. The column is well suited for the purification and analysis of sensitive DNA or RNA-based oligonucleotide products.
The column also provides exceptional packed bed stability over repeated conditions of increased temperatures and high pH conditions compared to silica-based columns of similar chemistry.
Learn more about the chemistry of our products for Oligonucleotide analysis through our website that also enables you to shop for lab equipment. You may want to explore the MassPREP Oligonucleotide Standard, which is designed for verification of HPLC/UPLC instrument and column performance for the analysis of synthetic oligonucleotides.
