ACQUITY UPLC CSH C18 Column, 130Å, 1.7 µm, 3 mm X 100 mm, 1/pk
In response to input collected at various customer forums, Waters developed the ACQUITY UPLC CSH C18 Column. The ACQUITY UPLC CSH C18 Column was designed to provide alternate selectivity compared to other reversed-phase UPLC columns. These ultra-efficient columns overcome peak shape asymmetry and poor loading capacity for basic compounds, column bleed, and rapid mobile-phase re-equilibration. The main mechanism behind the ACQUITY UPLC CSH columns is the Charged Surface Hybrid (CSH) Technology which is the next evolution of particle technology. Waters developed the Charged Surface Hybrid (CSH) Technology, the main mechanism behind the ACQUITY UPLC CSH columns.
The ACQUITY UPLC CSH C18 Column provides alternate selectivity, wider in range and different from Waters' other ACQUITY brands, ACQUITY UPLC BEH and XBridge. Without compromising necessary performance attributes such as peak shape for basic compounds, low column bleed, seamless transferability between different particle sizes and column formats, the ACQUITY UPLC CSH C18 Column offers a wide range of selectivities.
Since CSH Technology gives Trifluoroacetic acid (TFA) peak shape performance without the use of TFA, method development is faster and more straightforward using the ACQUITY UPLC CSH C18 Column. Though the use of Trifluoroacetic Acid means sharp peak shapes for basic compounds, it is an ion-pairing agent that is difficult to remove from the ACQUITY phase once a Liquid Chromatography column is exposed to it. In contrast, the ACQUITY UPLC CSH C18 Column is able to deliver improved peak shape performance with MS-friendly mobile phase additives such as formic acid. This also results in the faster and more straightforward method development as formic acid does not show effects of an ion-pairing agent. Additionally, the lab equipment provides increased analytical loadability, delivering sharper, narrower peaks for basic compounds under the acidic, low ionic strength mobile phase conditions. The columns are also ideally suited for isolation and purification as the increased loadability allows for the use of a narrower preparative column, reducing solvent consumption and fraction volumes.
To learn more about the technology and manufacturing of the ACQUITY UPLC CSH C18 Column, visit our website which also allows you to shop for lab equipment such as the ACQUITY UPLC CSH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk.
