Chemistry |
Amide |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
11 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Parker-style |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L68 |
Inner Diameter |
2.1 mm |
Length |
30 mm |
Carbon Load |
12 % |
eCord |
Yes |
UNSPSC |
41115709 |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC BEH AMIDE 1.7um 2.1x30mm
Using the ACQUITY UPLC BEH Amide Column, achieve remarkable retention of polar analytes across a broad range of polarity, structural moiety, and pKa. By utilizing Waters' cutting-edge stationary phases for Hydrophilic Interaction Chromatography (HILIC), which solves the problem of keeping and separating very polar substances, the columns enable this. The lab equipment utilizes a chemically stable, trifunctionally linked amide phase that adds stability and versatility for HILIC separations. This technique is based on ethylene bridging hybrid (BEH) particle technology.
For the analysis of carbohydrates, the ACQUITY UPLC BEH Amide Columns are excellent. They are the perfect lab equipment for the analysis of one of the most prevalent and diverse classes of compounds because their 1.7 um particle size ensures that high-speed and high-resolution analysis of carbohydrates is achieved in complex sample matrices while improving or maintaining chromatographic resolution.
Use the ACQUITY UPLC BEH Amide Columns to collapse reducing sugar anomers at high pH and high temperatures to speed up analysis and enhance MS detection.
The ACQUITY UPLC BEH Amide Columns also provide orthogonal selectivity and direct compatibility with sample preparation eluates as compared to reversed-phase materials (PPT, LLE, and SPE). You gain from improved retention of polar molecules as well as improved mass spectrometry responsiveness. You can find more Waters products to suit your analysis needs by browsing through our website, which also enables you to shop for lab equipment directly from us.
We recommend that you also use the ACQUITY UPLC BEH Amide VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk in conjunction with your ACQUITY column to ensure that it lasts a long time and that it continues to perform at its optimum throughout its lifetime. The pre-column is manufactured using the exact same materials as those present in the column to ensure there is no loss of efficiency. It works by eliminating any unwanted contaminants from the mobile stream.
Across What pH Range Does The ACQUITY UPLC BEH AMIDE Column Operate?
The ACQUITY UPLC BEH AMIDE column listed here is able to function well within the 2-11 pH range.
What Is The Maximum Pressure That The ACQUITY UPLC BEH AMIDE Column Can Tolerate?
This particular ACQUITY UPLC BEH AMIDE Column is able to tolerate a maximum of 18000 psi (1240 Bar) pressure.