ACQUITY UPLC BEH Amide Column, 130Å, 1.7 µm, 3 mm X 150 mm, 1/pk
Choose the ACQUITY UPLC BEH Amide Column to benefit from the chemically stable, tri-functionally linked amide phase based on Waters' innovative ethylene bridged hybrid (BEH) particle technology. For HILIC (Hydrophilic Interaction Chromatography) separations, this adds a new level of stability and adaptability. The use of unbonded BEH particles in laboratory equipment also allows for orthogonal separation of polar and ionizable chemical mixtures. The column has been designed and proven to achieve efficient, repeatable separations under UPLC HILIC settings, with a unique design that helps to overcome one of the HILIC stationary phases' primary flaws, chemical stability.
To obtain great retention of polar analytes across a wide range of polarity (including carbohydrates and sugars), structural moiety, and pka, add the ACQUITY UPLC BEH Amide Column to your lab equipment collection. The column may be used with a wide range of mobile phase pH since it was developed to retain polar analytes and metabolites that are too polar for reversed-phase (RP) chromatography (2-11). The combination of BEH technology and a tri-functionally bonded amide phase in the column results in a column with remarkable lifetime and assay robustness.
The ACQUITY UPLC BEH Amide Column is an excellent choice for lab equipment because it provides increased mass spectrometry response, direct compatibility with sample preparation elutes (PPT, LLE, and SPE), and orthogonal selectivity compared to reversed-phase materials.
To do excellent carbohydrate analysis, use the ACQUITY UPLC BEH Amide Column. The column's 1.7 µm particle size allows for high-speed and high-resolution analysis in complicated matrices while enhancing or preserving chromatographic resolution. It also enhances quantitation accuracy because, unlike amine-based columns, it is not vulnerable to Schiff base formation. The ACQUITY UPLC BEH Amide Column's higher pH stability allows for the use of high pH and high temperature to collapse, minimizing sugar anomers, lowering analysis times, and increasing MS detection.
Visit our website to shop for lab equipment and contact us with any questions you may have. You may also be interested in ACQUITY UPLC BEH Amide VanGuard Pre-column; Amide columns, such as ACQUITY BEH, hold very polar molecules such as carbohydrates and sugars. The ACQUITY Amide columns, which are based on BEH technology, have a chemically stable, tri-functionally linked amide phase that is stable from pH 2 to 12, allowing them to separate polar analytes with a wide range of polarity, structural moiety, and pKa.
