SKU: 186004742
ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1K - 150K, 1/pk

ACQUITY UPLC Glycan BEH Amide Columns | 2.1mm X 150mm | 186004742

Product Description

The ACQUITY UPLC BEH Glycan Separation Technology Column, also known as BEH Glycan, is optimized for use with ACQUITY UPLC systems with fluorescence detection (FLR), separating the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives.

Specifications

  • UNSPSC

    41115709

  • Application

    Glycan

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

  • Carbon Load

    12 %

  • Chemistry

    Amide

  • Endcapped

    No

  • Endfitting Type

    Parker-style

  • Format

    Column

  • Inner Diameter

    2.1 mm

  • Length

    150 mm

  • Separation Mode

    Hydrophilic Interaction (HILIC)

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    150000

  • Particle Shape

    Spherical

  • Particle Size

    1.7 µm

  • Particle Substrate

    Hybrid

  • Pore Size

    130 Å

  • QC Tested

    Glycan

  • Silanol Activity

    Low

  • Surface Area

    185

  • System

    UHPLC, UPLC

  • Particle Technology

    BEH

  • USP Classification

    L68

  • pH Range Min

    2 pH

  • pH Range Max

    11 pH

What are glycans?

Glycan is another term for a polysaccharide, or a compound consisting of a large number of monosaccharides like glycosidically. The term glycan can also be used to refer to the carbohydrate portion of a glycoconjugate. Glycosylation is one of the most common forms of post-translational modification of human proteins. Glycosylated proteins make up 50-70% of human proteins, while about 30% of the cell surface is made up of glycolipids and glycoproteins. Glycans, therefore, play critical roles in many physiological and pathological reactions, including immunity, blood clotting, cell development, and death.

What is UPLC?

UPLC stands for Ultra Performance Liquid Chromatography. It is the next step in separation science beyond HPLC, or High Performance Liquid Chromatography. UPLC Columns are packed with smaller particles [1.7 – 1.8 µm], enabling your lab to achieve the highest level of chromatographic performance. With UPLC Technology, your lab can improve resolution, sensitivity and speed, all without compromise.

ACQUITY UPLC Glycan BEH Amide Column, 130Å, 1.7 µm, 2.1 mm X 150 mm, 1/pk

Specifically developed and extensively QC tested for superior UPLC component resolution in less time for glycan structures, the ACQUITY UPLC BEH Glycan column works with Waters’ ACQUITY UPLC system to achieve column chemistry that is suited ideally to help chromatographers get the correct answers more quickly. Waters’ Glycan separation technology is optimized for use with ACQUITY UPLC systems with fluorescence detection, offering improved component resolution in a shorter time than other HPLC-based methods.

Waters’ glycan analysis UPLC columns are based on the patented second-generation BEH hybrid particles. With this proprietary bonding process, Waters has developed a robust, amide based, ligand chemistry to be used in the demanding application of glycan separation. Extensive quality control testing is performed against relevant, labeled glycan standards in order to ensure consistent column to column performance and batch to batch. This state of the art bonding procedure for short amine-containing liquid is combined with the particle structure and bonding chemistry that remains stable at low pH and elevated temperature to offer consistent glycan separations.

By combining the ACQUITY UPLC system with BEH technology with this technology for glycan separation, analysis time and cost per sample can be reduced while resolution is improved. By using several complementary analytical techniques, scientists are able to better characterize, identify, and quantitate oligosaccharides isolated from glycoproteins. HLIC is a well recognized, reliable technique that can be effectively used to separate fluorescently-labeled glycan. In combination with Waters’ ACQUITY UPLC system, the ACQUITY UPLC BEH Amide Column gives improved component resolution compared to more conventional LC techniques, offering more certain identification and reliable quantitation.

Each batch of BEH Glycan column material undergoes rigorous testing to ensure consistent and reliable performance. All columns include a Certificate of Analysis which reports physical, chemical, and chromatographic tests obtained from the actual separation of a qualified 1-AB Labeled Human IgG standard.

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