Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Temperature Limits |
45 C |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
No |
Bonding Technology |
C18 SB |
Silanol Activity |
Medium |
Particle Shape |
Spherical |
Particle Size |
1.8 µm |
Endfitting Type |
Parker-style |
Pore Size |
100 Å |
Format |
Column |
Surface Area |
230 |
System |
UPLC, UHPLC |
Particle Technology |
HSS |
USP Classification |
L1 |
Inner Diameter |
3 mm |
Length |
150 mm |
Carbon Load |
8.5 % |
eCord |
Yes |
UNSPSC |
41115709 |
Brand |
ACQUITY UPLC |
Product Type |
Columns |
Units per Package |
1 pk |
ACQUITY UPLC HSS C18 SB Column, 100Å, 1.8 µm, 3 mm X 150 mm, 1/pk
For low pH separations containing complicated combinations of basic and non-basic chemicals, ACQUITY UPLC HSS C18 SB columns are employed. Compared to the majority of contemporary, high coverage, completely end-capped C18 stationary phases, ACQUITY HSS C18 SB columns offer alternate analyte selectivity. Fully scalable and transferrable to HPLC separations are the ACQUITY HSS C18 SB columns.
Reduce the likelihood of technique variation brought on by inconsistent batches of chromatographic medium or columns. This is why you can count on the ACQUITY UPLC HSS C18 SB Column to offer reliable results year after year because it was designed to produce predictable and reproducible results.
Utilize the distinct non-end-capped, low-coverage silica-based C18 chemistry of the ACQUITY UPLC HSS C18 SB Column to achieve alternative selectivity for compounds affected by silanophilic interactions. The lab apparatus has a specific increased silanol activity as a result of secondary interactions with leftover silanols, which increases the retention of fundamental chemicals. This happens as ionic repulsion, and poor ligand density reduces non-basic analyte retention. The unique mix of retention properties found in the ACQUITY UPLC HSS C18 SB Column makes it a powerful method development tool capable of delivering modifications in Selectivity for Bases (SB) while providing excellent peak shape.
The packaging materials for the ACQUITY UPLC HSS C18 SB Column were created exclusively for the ACQUITY UPLC System. Additionally, they are made with ultra-pure reagents in a facility that is ISO 9001 and cGMP certified. To ensure exceptional, repeatable performance, each batch of the packing material is chromatographically tested with acidic, basic, and neutral analytes, and the results are held to narrow specification ranges.
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We recommend reviewing ACQUITY UPLC HSS C18 SB VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 3/pk; For low pH separations containing complicated combinations of basic and non-basic chemicals, ACQUITY UPLC HSS C18 SB columns are employed. Compared to the majority of contemporary, high-coverage, completely end-capped C18 stationary phases, ACQUITY HSS C18 SB columns offer alternate analyte selectivity. Fully scalable and transferrable to HPLC separations are the ACQUITY HSS C18 SB columns. By immediately connecting to the ACQUITY UPLC column's intake, the VanGuard pre-column decreases the volume of additional columns and reduces leaking by doing away with superfluous connections.
Why Do Cations Only Appear in Data from Mass Spectrometry?
Only ions can be recognized when measuring the mass of charged particles using mass spectrometry since neutral molecules are invisible.