The XBridge Protein BEH C4, 300 Å, 3.5 µm, 4.6 mm x 100 mm column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.
Chemistry |
C4 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
2 pH |
pH Range Max |
10 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
No |
Silanol Activity |
Low |
Molecular Weight Range Max |
150000 |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
300 Å |
QC Tested |
Protein |
Format |
Column |
Surface Area |
90 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L26 |
Inner Diameter |
4.6 mm |
Length |
100 mm |
Carbon Load |
8 % |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Protein BEH C4 Column, 300Å, 3.5 µm, 4.6 mm X 100 mm, 10K - 500K, 1/pk
Use the XBridge Protein BEH C4 Column, which is part of the Waters Protein Separation Technology (PrST) Columns, that are QC tested and optimized to separate proteins based on size, hydrophobicity, and isoelectric point. BEH technology is used in the lab equipment, and synthetic particles are used to provide the highest quality and constant performance. If you compare traditional to C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery and improve peak capacity.
The XBridge Protein BEH C4 Column provides excellent peptide separation for a wide range of peptides. For maximum resolution, it also produces thin and symmetrical peaks. The XBridge Protein BEH C4 Column provides excellent peak shape and retention in formic acid and trifluoroacetic acid for optimum chromatography.
Browse our catalog for more variations of the product featured here, as well as products that are compatible with it, and shop for lab equipment. It will also allow you to call our offices across the world and speak with members of our team if you have any questions about the products or technology.
To assure the stability of peptide separation procedures, the XBridge Protein BEH C4 Column is QC verified with a peptide map. This is made achievable by using well-characterized, cutting-edge C18 ligand bonding techniques. Furthermore, it ensures consistent results with a wide range of samples utilized in proteomics, protein characterization, and peptide synthesis. The analytical column that results provides consistent synthetic peptide and protein digest separations from batch to batch. The lab equipment's manufacturing procedures enable stable particle structure and bonding chemistry across a wide pH and at high temperatures.
We recommend that you also invest in the LCGC Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk. To acquire trustworthy and interference-free results from your analysis, store your analytes, samples, and solutions in the chemically inert vial. All Waters LC/GC certified vials are also UV-tested by HPLC, a test that detects trace quantities of contaminants used in packaging and manufacture.
Across What Range Does The XBridge Protein BEH C4 Column Operate?
The XBridge Protein BEH C4 Column listed here offers a wide operating pH range of 2-12.
What Maximum Pressure Does The XBridge Protein BEH C4 Column Offer?
The XBridge Protein BEH C4 Column is able to work with up to 6000 psi (415 Bar) pressure.