SKU: 186004502
XBridge Protein BEH C4 Column, 300 Å, 3.5 µm, 4.6 mm x 50 mm, 1/pk

XBridge Protein BEH C4 Column | 186004502


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Product Description

The XBridge Protein BEH C4, 300 Å, 3.5 µm, 4.6 mm x 50 mm column is QC tested and optimized to separate proteins based on size, hydrophobicity and isoelectric point. If you compare to traditional C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery, and improve peak capacity.

Specifications

  • Chemistry

    C4

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    2 pH

  • pH Range Max

    10 pH

  • Maximum Pressure

    6000 psi (415 Bar)

  • Endcapped

    No

  • Silanol Activity

    Low

  • Molecular Weight Range Max

    150000

  • Particle Shape

    Spherical

  • Particle Size

    3.5 µm

  • Endfitting Type

    Waters

  • Pore Size

    300 Å

  • QC Tested

    Protein

  • Format

    Column

  • Surface Area

    90

  • System

    HPLC

  • Particle Technology

    BEH

  • USP Classification

    L26

  • Inner Diameter

    4.6 mm

  • Length

    50 mm

  • Carbon Load

    8 %

  • UNSPSC

    41115709

  • Application

    Protein

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XBridge Protein BEH C4 Column, 300Å, 3.5 µm, 4.6 mm X 50 mm, 10K - 500K, 1/pk

The lab equipment's wide-pore (300) tri-functionally bound BEH particle can also be used for protein characterization and peptide synthesis. The XBridge Protein BEH C4 Column based on Peptide Separation Technology (PST) has been optimized and QC confirmed for peptide separations. Waters Protein Separation Technology (PST) columns are QC tested and optimized to separate proteins based on size, hydrophobicity, and isoelectric point. If you compare traditional to C18 phases, the C4 ligand is less retentive and will minimize protein carryover, increase protein recovery and improve peak capacity.

To take advantage of the Protein Separation Technology of the analytical column, which integrates BEH technology and uses synthetic particles to deliver the best quality and consistent performance, add the XBridge Protein BEH C4 Column to your lab equipment collection.

The XBridge Protein BEH C4 Column is QC confirmed with a peptide map to ensure the stability of peptide separation procedures. This is made possible by utilizing well-known, cutting-edge C4 ligand bonding techniques, which provide consistent results across a wide range of proteomics, protein characterization, and peptide synthesis data. As a result, you can count on the analytical column's consistency to produce consistent synthetic peptide and protein digest separations from batch to batch. Particle structure and bonding chemistry are stable from pH 2 to 12 and up to 6,000 psi thanks to the techniques used in lab equipment production (or 415 Bar).

The packing materials for the XBridge Protein BEH C4 Columns were developed in a cGMP and ISO 9002 recognized facility using an ultra-pure reagent to ensure good peak shape, efficiency, and stability. Each batch of XBridge Peptide BEH C18 Column material undergoes a peptide separation, with the findings held to specific ranges. This is done to ensure high-quality, repeatable peptide separation. To learn more about additional product offerings from Waters, similar and compatible to the XBridge Protein BEH C4 Column listed here, please browse through our website or brochure to shop for lab equipment so you can replenish your stock and add to your existing equipment portfolio.

We also recommend that you look into the Cytochrome C Digestion Standard, which was made by digesting Bovine Heart Cytochrome C (Uniprot # P62894) with sequencing grade trypsin. Each batch of digestion standard is made under tight quality control guidelines, as evidenced by the certificate of analysis that comes with it. Protein digestion standards can be used for a variety of purposes, including as evaluating and benchmarking HPLC and UPLC columns, as well as assessing the performance of mass spectrometry and chromatography instruments.

What Is The Recommended pH Range For TheXBridge Protein BEH C4 Column?

The XBridge Protein BEH C4 Column can easily operate within a pH range of 2 to 12. However, you should note that working at the extremes of pressures, pH, and/or temperatures will result in shorter column lifetimes.