Chemistry |
HILIC |
Separation Mode |
Hydrophilic Interaction (HILIC) |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
9 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L3 |
Inner Diameter |
2.1 mm |
Length |
100 mm |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge BEH HILIC Column, 130Å, 3.5 µm, 2.1 mm X 100 mm, 1/pk
Benefit from the unbonded BEH substrates that are present in the Waters XBridge BEH HILIC columns, as they enable you to retain and separate very polar compounds that are not well retained by traditional reversed-phase chromatography. The BEH particle’s chemical stability results in long column lifetimes as it is more rugged than silica-based HILIC phases. The unique lab equipment is optimized and tested to produce efficient, reproducible separations under HPLC HILIC conditions.
Using the XBridge BEH HILIC Column, you can leverage alternative selectivity compared to traditional reversed-phase columns. In addition to this, the lab equipment is able to provide improvements in peak shape, retention, and column lifetime when compared to silica-based HILIC columns. The robust and rugged BEH Technology the XBridge BEH HILIC Column is based on makes the lab equipment a new standard for HILIC separations. The analytical column makes this possible by the unique attributes of Hydrophilic Interaction Chromatography (HILIC).
Getting enhanced retention for polar analytes is made easy with the use of the XBridge BEH HILIC Column. The presence of the HILIC technology in the column enables complex retention mechanisms that combine partitioning, ion exchange, and hydrogen bonding. The XBridge Column also gives you greater retention than reversed-phase, as retention in this column occurs with a polar stationary phase combined with mobile phases made up of acetonitrile concentrations greater than 80%. The analytical column also offers complimentary selectivity to reversed-phase columns. Further, in addition to improved retentivity, reversal in elution has also been observed by the use of the XBridge BEH HILIC Column. This is especially beneficial when developing orthogonal separations for polar analytes.
The XBridge BEH HILIC Column is available in different variations as well. You can find more about the chemistry of the column using our catalog or technology guides available on our website.
To ensure that your XBridge analytical column lasts a long time, and continues to perform at its optimal level, invest in the XBridge BEH HILIC VanGuard Cartridge, 130Å, 3.5 µm, 2.1 mm X 5 mm, 3/pk. The lab equipment is designed to protect your column from harm by removing all chemical and particulate contaminants from reaching the mobile phase.
What Is The pH Range For The XBridge BEH HILIC Column?
The XBridge BEH HILIC Column listed here offers a pH range of 1-9.