Chemistry |
Phenyl |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
18000 psi (1240 Bar) |
Endcapped |
Yes |
Bonding Technology |
Phenyl-Hexyl |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
1.7 µm |
Endfitting Type |
Direct-Connect |
Pore Size |
130 Å |
Format |
VanGuard Pre-column |
Surface Area |
185 |
System |
UHPLC, UPLC |
Particle Technology |
BEH |
USP Classification |
L11 |
Inner Diameter |
2.1 mm |
Length |
5 mm |
Carbon Load |
15 % |
UNSPSC |
41115709 |
Brand |
ACQUITY UPLC |
Product Type |
Guards |
Units per Package |
3 pk |
ACQUITY UPLC BEH Phenyl-Hexyl VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk
With the complementary selectivity of ACQUITY BEH Phenyl Columns and the use of XBridge BEH Phenyl, ACQUITY UPLC BEH Phenyl-Hexyl columns provide chromatographers with the most chemically stable phenyl sorbent on the market for ultra-low column bleed, long column lifetimes, and excellent peak shapes. When working with industry best lab equipment and columns, you will want to ensure you minimize extra column volumes and reduce leakage by eliminating additional connections. VanGuard Pre-columns were designed to prevent these problems while maintaining the benefits of the column used.
The VanGuard line of Pre-columns are made to prolong analytical column lifetimes without compromising the chromatographic performance of the columns. Made specifically to match your desired chemistry, ACQUITY UPLC BEH Phenyl-Hexyl VanGuard Pre-columns are made to work with the corresponding columns. Once attached, the Pre-columns can remove particulate and chemical contamination while maintaining UPLC, UHPLC, and HPLC separation efficiency. This cost-effective tool provides protection for analytical columns, making them last longer and for more separations.
As the first guard column for UPLC and UHPLC applications, the ACQUITY UPLC BEH Phenyl-Hexyl VanGuard Pre-column offers guaranteed compatibility with pressures up to 18,000 psi, while the patent-pending ultra-low volume design creates minimal chromatographic effects. All Pre-columns are manufactured using Waters’ column hardware, particles, and sorbent chemistries for superior column protection and performance.
The tool can be used to avoid leaks and connection voids with ACQUITY UPLC BEH C18 VanGuard Pre-column, 130Å, 1.7 µm, 2.1 mm X 5 mm, 3/pk and other similar columns in the same family.
When Should I Replace My ACQUITY UPLC BEH Phenyl-Hexyl VanGuard Pre-Column?
Waters recommends all guard columns are replaced before contaminants can negatively affect the analytical column. Indications of contamination include: changes in peak shape, peak splitting, shoulders on the peak, shifts in retention, change in resolution, or increasing backpressure. To avoid these issues, replace the guard column at regular intervals determined based on method performance. You may choose to change after running 50 to 100 samples, on a weekly basis, or after completion of a set of analyses for example. If contaminants appear in your chromatogram, replace immediately and follow recommendations for cleaning the column.