XBridge Peptide BEH C18 Column, 130Å, 10 µm, 4.6 mm X 250 mm, 1/pk

For peptide separations, the XBridge BEH130 C18 Peptide Separation Technology (PST) columns have been optimized and QC verified. These columns are particularly evaluated using a peptide map to ensure scientists acquire stable peptide separation methods as well as predictable behavior with a variety of different samples that it may be used for—such as proteomics, protein characterization, and peptide synthesis

The column's injected BEH Technology will improve the resolution of complex peptide digests. The XBridge Peptide BEH C18 Column provides good separation across a wide range of peptides. It also improves peak shape and retention in formic acid and trifluoroacetic acid for better chromatography. You will also be able to migrate from sub-2 µm UPLC Technology to 10 µm preparative HPLC separations using the XBridge Peptide BEH C18 Columns.

The XBridge Peptide BEH C18 Column is delivered in acetonitrile. Before switching to a different mobile phase system, it is critical to establish mobile phase compatibility. We strongly advise equilibrating the column with at least 10 column volumes of the mobile phase to be utilized. To prevent mobile phase buffers from precipitating out on your column or in your system, flush the column with another five-column volume of a water/organic solvent mixture with the same or lower solvent content as your desired buffered mobile phase.

To improve selectivity, minimize solvent viscosity, and increase mass transfer rates, the XBridge Peptide BEH C18 Column should be operated at temperatures ranging from 20 to 80 degrees Celsius. The XBridge Peptide BEH C18 Column can withstand pressures of up to 6000 psi (or 415 bar). We recommend maintaining pressure and temperature within the ambient range, as anything over the ranges will have a negative effect on the lifetime of the column depending on the pH and buffer conditions utilized.

Visit our website to learn about other XBridge columns and to shop for lab equipment as per your lab needs. You may also be interested in the XBridge Peptide BEH C18 OBD Prep Column, 130Å, 10 µm, 10 mm X 250 mm, 1/pk, which has a small-pore (130) tri functionally bonded BEH particle that provides the widest useable pH range, excellent low pH stability, and ultra-low column bleed to test samples for proteomics, protein characterization, and peptide synthesis.