SKU: 186003621
XBridge Peptide BEH C18 Column, 300Å, 5 µm, 2.1 mm X 250 mm, 1K - 15K, 1/pk

XBridge Peptide BEH C18 Column | 186003621


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Product Description

The XBridge BEH300 C18 Peptide Separation Technology (PST) columns are optimized and QC tested for peptide separations. The wide-pore (300Å) trifunctionally bonded BEH particle offers you the widest usable pH range, superior low pH stability, and ultra-low column bleed to assay samples for proteomics, protein characterization, and peptide synthesis.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Hybrid

  • pH Range Min

    1 pH

  • pH Range Max

    12 pH

  • Maximum Pressure

    6000 psi (415 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Molecular Weight Range Min

    1000

  • Molecular Weight Range Max

    15000

  • Particle Shape

    Spherical

  • Particle Size

    5 µm

  • Endfitting Type

    Waters

  • Pore Size

    300 Å

  • QC Tested

    Peptide

  • Format

    Column

  • Surface Area

    185

  • System

    HPLC

  • Particle Technology

    BEH

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    250 mm

  • Carbon Load

    18 %

  • UNSPSC

    41115709

  • Application

    Peptide

  • Brand

    XBridge

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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XBridge Peptide BEH C18 Column, 300Å, 5 µm, 2.1 mm X 250 mm, 1K - 15K, 1/pk

With low pH stability and extremely little column bleed, the XBridge Peptide BEH C18 Column allows you to analyze samples for proteomics over a broad pH range. The wide-pore (300) tri-functionally bound BEH particle used in the lab apparatus can also be used for protein characterization and peptide synthesis. The XBridge BEH300 C18 Peptide Separation Technology (PST) columns have been QC tested and optimized for peptide separations.

The XBridge Peptide BEH C18 Column underwent quality control verification using a peptide map to ensure the stability of peptide separation procedures. This is accomplished by utilizing established, state-of-the-art bonding procedures for the C18 ligand, which provide reproducible outcomes for a variety of samples utilized in proteomics, protein characterization, and peptide synthesis. You may depend on the analytical column's consistency; as a result, you will get consistent separations of synthetic peptides and protein digests from batch to batch.

A cGMP, ISO 9002 accredited plant produced the packing materials for the XBridge Peptide BEH C18 columns using an ultra-pure reagent to produce good peak shape, high efficiency, and exceptional stability. Each batch of XBridge Peptide BEH C18 Column material undergoes a peptide separation; the results are held to precise specification ranges. To achieve high, reproducible peptide separation performance, this is done. Each column is tested, and a Performance Test Chromatogram, as well as a Certificate of Acceptance, are included in the package with each column.

To benefit from the Protein Separation Technology of the analytical column, which incorporates BEH technology and uses synthetic particles to deliver the best quality and consistent performance, add the XBridge Peptide BEH C18 Column to your collection of lab supplies. Discover more Waters products and collections on our website, and shop for lab equipment based on the requirements of your lab.

You might want to look into TruView LCMS Certified Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and Preslit PTFE/Silicone Septum, 2 mL Volume, 100/pk; Vials that are TruView LCMS Certified are made using strictly regulated manufacturing and handling techniques. Mass spectrometers are used to check for cleanliness, and ACQUITY/MS/MS is used to check for low analyte adsorption.

How Does BEH Technology Work?

The BEH (Ethylene Bridged Hybrid) particle, one of the fundamental components of UPLC technology, is available in a range of pore diameters and bonded phases, enabling both reversed-phase and hydrophilic contact chromatography. The method is applicable to a variety of biopharmaceutical analyses, including small molecules to big molecules. A larger range of usable pH values is made possible by hybrid particle technology's inherent chemical stability, which also makes it a flexible, reliable separation technology for method development.