Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
15000 |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
QC Tested |
Peptide |
Format |
Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
2.1 mm |
Length |
50 mm |
Carbon Load |
17 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge Peptide BEH C18 Column, 130Å, 3.5 µm, 2.1 mm X 50 mm, 1K - 15K, 1/pk
Peptide separations are adjusted and quality-controlled evaluated using the XBridge BEH130 C18 Peptide Separation Technology (PST) columns. To evaluate samples for proteomics, protein characterization, and peptide synthesis, the small-pore (130) tri-functionally bonded BEH particle offers you the widest usable pH range, improved low pH stability, and ultra-low column bleed. Your productivity can go up if you use the XBridge BEH C18 XP Column. The XBridge BEH C18 Column is a top-notch piece of laboratory gear whether you are developing a cutting-edge LC/MS assay or a quality control procedure.
The column will improve the resolution of the complex peptide digests with the addition of BEH Technology. Your choice of peptides will be effectively separated using the XBridge Peptide BEH C18 Column. For the best chromatography, it will also provide you with improved peak shape and retention in formic acid and trifluoroacetic acid. With The XBridge Peptide BEH C18 Columns, you may seamlessly transition from sub-2 m UPLC Technology to 10 m preparative HPLC separations.
Operating the XBridge Peptide BEH C18 Column at temperatures between 20 and 80 degrees Celsius is advised in order to improve selectivity, reduce solvent viscosity, and boost mass transfer rates. While under pressure, the XBridge Peptide BEH C18 Column can withstand 6000 psi (or 415 bar). Depending on the pH and buffer conditions employed, we advise keeping pressure and temperature within the ambient range. Anything outside of those ranges will reduce the column's lifetime.
The XBridge BEH C18 Column is constructed in specialized Waters chromatography production factories under the strictest cGMP, ISO 9002 guidelines, much like all other products made by the company. The tightly regulated atmosphere in the manufacturing facilities guarantees that you will obtain lab equipment of the highest caliber with accurate and repeatable test results.
You may shop for lab equipment on our website and in our catalog, where you can also examine our wide selection of XBridge columns for a number of applications. Any inquiries or concerns can be sent to a member of our international staff, and we would be happy to help.
We also recommend reviewing XBridge BEH C18 XP VanGuard Cartridge, 130Å, 2.5 µm, 2.1 mm X 5 mm, 3/pk; By eliminating particle pollution from the mobile phase stream, XBridge BEH C18 VanGuard Cartridges are utilized to increase analytical column lifetime and performance. All 2.1 mm and 3.0 mm I.D. XBridge BEH C18 analytical columns with 2.5 µm sorbent particles are protected by this cartridge.
What pH Range Is Best For XBridge Peptide BEH C18?
The optimal pH range for the XBridge Peptide BEH C18 is between 1 and 12. Please be aware that utilizing a higher pH may affect the column's lifespan and functionality.