Columns for Antibody Drug Conjugates (ADC)

SKU: 186003532
ACQUITY UPLC HSS C18 Column, 100Å, 1.8 µm, 2.1 mm X 50 mm, 1/pk

ACQUITY UPLC HSS C18 Columns | 186003532


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Product Description

ACQUITY UPLC HSS C18 chemistry is an ultra-performance general purpose C18 bonded phase that provides superior peak shape for bases, increased retention (vs. ACQUITY UPLC BEH C18 columns), and extremely long column lifetimes at low pH. ACQUITY HSS C18 columns are fully scalable and transferable to HPLC separations.

Specifications

  • Chemistry

    C18

  • Separation Mode

    Reversed Phase

  • Particle Substrate

    Silica

  • pH Range Min

    1 pH

  • pH Range Max

    8 pH

  • Temperature Limits

    45 C

  • Maximum Pressure

    18000 psi (1240 Bar)

  • Endcapped

    Yes

  • Silanol Activity

    Low

  • Particle Shape

    Spherical

  • Particle Size

    1.8 µm

  • Endfitting Type

    Parker-style

  • Pore Size

    100 Å

  • Format

    Column

  • Surface Area

    230

  • System

    UHPLC, UPLC

  • Particle Technology

    HSS

  • USP Classification

    L1

  • Inner Diameter

    2.1 mm

  • Length

    50 mm

  • Carbon Load

    15 %

  • eCord

    Yes

  • UNSPSC

    41115709

  • Brand

    ACQUITY UPLC

  • Product Type

    Columns

  • Units per Package

    1 pk

Product Support

Documents

Documents



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ACQUITY UPLC HSS C18 Column, 100Å, 1.8 µm, 2.1 mm X 50 mm, 1/pk

Born from an innovative synthetic process, Waters’ High Strength Silica (HSS) particle technology increases the mechanical stability of silica while maintaining pore volumes similar to those in HPLC silica-based materials. This novel particle technology provides increased retentivity when compared to hybrid particles while serving as the ideal substrate to create stationary phases with alternate selectivity that can withstand UPLC pressures. The ACQUITY UPLC HSS C18 Column offers high mechanical stability and the appropriate morphology to provide long column lifetimes and UPLC efficiencies at pressures up to 15,000 psi (1000 bar). The 1.8 µm UPLC HSS particle is designed and tested specifically for use in UPLC separations.

The ACQUITY UPLC HSS C18 chemistry is a full end-capped, high coverage, ultra-performance C18 bonded phase which provides peak shape for bases, along with increased retention when compared to lab equipment like the ACQUITY UPLC BEH C18 Column. The stationary phase features a trifunctionally bonded C18 ligand in addition to a proprietary process for end-capping that yields superior peak shapes for bases at neutral pH, while also resisting acidic hydrolysis, allowing for extremely long lifetimes at low pH.

ACQUITY HSS packing materials are designed to be used with the ACQUITY UPLC system and related products like the ACQUITY UPLC HSS C18 VanGuard Pre-column, 100Å, 1.8 µm, 2.1 mm X 5 mm, 3/pk and will offer the best performance when used in this family. Each column is individually tested and includes a Performance Chromatogram and a Certificate of Batch analysis. Each batch of ACQUITY HSS material is also tested chromatographically with acidic, basic, and neutral analytes, with results held to narrow specification ranges.

Where Can I Get Column Use Information?

Each ACQUITY UPLC HSS C18 Column comes with an eCord chip that provides column use data. When used, the software will display identifiers of the column such as chemistry type, column dimensions, and serial number. Overall column usage information will include the total number of samples, total number of injections, total sample sets, date of first injection and last injection, maximum pressure, and temperature. This information will also detail the column history by sample set, including the date started, sample set name, user name, system name, number of injections and samples in the sample set, maximum pressure, and temperature.