Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Hybrid |
pH Range Min |
1 pH |
pH Range Max |
12 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Silanol Activity |
Low |
Particle Shape |
Spherical |
Particle Size |
5 µm |
Endfitting Type |
Waters |
Pore Size |
130 Å |
Format |
Column |
Surface Area |
185 |
System |
HPLC |
Particle Technology |
BEH |
USP Classification |
L1 |
Inner Diameter |
3 mm |
Length |
100 mm |
Carbon Load |
18 % |
UNSPSC |
41115709 |
Brand |
XBridge |
Product Type |
Columns |
Units per Package |
1 pk |
XBridge BEH C18 Column, 130Å, 5 µm, 3 mm X 100 mm, 1/pk
Utilize the XBridge BEH C18 Column for better low and high pH stability to obtain outstanding chromatographic peaks. By leveraging its patented trifunctional bonding and end-capping technology, the Waters XBridge BEH C18 Column avoids the low-pH instability brought on by siloxane bond cleavage via acid hydrolysis and instead restricts the rate of hydrolysis. This leads to a lower retention loss than columns filled with monofunctional silane phases. This holds true even in accelerated low-pH test environments.
In the development of HPLC methods, the XBridge BEH C18 sorbent can be used over the whole pH range of the mobile phase. The XBridge BEH C18 Column's C18 ligand, on which it is based, is trifunctionally linked to provide a column that can be used for the majority of HPLC separations and has the widest pH range, the best low pH stability, and the least amount of column bleed.
By preventing particle dissolving, the ethylene bridges found inside the silica matrix of the XBridge BEH C18 Column particles solve the problems associated with silica-based chromatographic particle failure at high pH, which causes column voids, efficiency loss, and peak degradation.
The XBridge BEH C18 Columns offer unmatched freedom in method creation. The lab equipment, which is produced in cGMP, ISO9001, and ISO13485 certified facilities where every step is meticulously scrutinized for quality, offers exceptional reproducibility year after year, removing the need to test each new batch of columns and retaining time-honored procedures. The XBridge BEH C18 Columns deliver accurate results and consistent column performance.
You may also be interested in learning more about the XBridge BEH C18 VanGuard Cartridge, 130Å, 5 µm, 2.1 mm X 5 mm, 3/pk, which has been designed especially for the XBridge BEH C18 Column and made using the exact same packing materials. The function of the cartridge is to protect the column from harm by eliminating the presence of chemical or particulate contaminants.
What Is The pH Across Which The Waters XBridge BEH C18 Column Works?
The Waters XBridge BEH C18 Column listed here works well across the 1-12 pH range.
What Is The Maximum Pressure That The Waters XBridge BEH C18 Column Can Tolerate?
The XBridge BEH C18 Column is able to take up to 6000 psi (415 Bar) pressure.