Primarily supports legacy methods. BioSuite Peptide Analysis columns consist of two premier reversed-phase column chemistries specifically optimized for peptide mapping from simple to complicated digests. BioSuite PA Columns are available in various configurations for LC or LC-MS applications, including 250 mm lengths for ultra high resolution. These BioSuite PA-A and PA-B columns provide excellent batch-to-batch reproducibility for consistent results and are uniquely QC tested specifically for peptide mapping. BioSuite C18 3 µm PA-A is a 100Å, difunctional bonded, low ligand density, silica-based reversed-phase column and BioSuite C18 3.5 µm PA-B is a 300Å, high ligand density, monofunctional, silica-based reversed-phase column.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
30000 |
Particle Shape |
Spherical |
Particle Size |
3.5 µm |
Endfitting Type |
Waters |
Pore Size |
300 Å |
Format |
Column |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
2.1 mm |
Length |
250 mm |
Carbon Load |
8.5 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
BioSuite |
Product Type |
Columns |
Units per Package |
1 pk |
BioSuite C18 PA-B Column, 300 Å, 3.5 µm, 2.1 mm X 250 mm, 1K - 30K, 1/pk
The BioSuite™ Peptide Analysis columns consist of two premier reversed-phase column chemistries specifically optimized for peptide mapping from simple to complicated digests. BioSuite PA Columns are available in various configurations for LC or LC/MS applications, including 250 mm lengths for ultra-high resolution. These BioSuite™ PA-A and PA-B columns provide excellent batch-to-batch reproducibility for consistent results and are uniquely QC tested specifically for peptide mapping. The lab equipment listed here is the BioSuite™ C18 3.5µm PA-B, a 300Å, high ligand density, monofunctional, silica-based reverse-phase column.
Opt for the use of the BioSuite C18 PA-B Column in order to get exceptional performance when you are separating complex digests that contain peptides that are hydrophilic, hydrophobic, as well as basic. The lab equipment is designed to easily provide superior peak shapes and capacity for peptide separations using TFA-containing eluents. In addition to this, it is a great option for the separation of larger peptide fragments generated by non-trypsin endoproteases.
The BioSuite C18 PA-B Column packing materials were created expressly for use with the ACQUITY UPLC System and are made in a cGMP, ISO 9001 certified facility with ultra-pure reagents. Each batch of BioSuite C18 PA-B Column packing material is chromatographically tested with acidic, basic, and neutral analytes, with results held within narrow specification ranges to ensure outstanding, repeatable performance. On the eCordTM Intelligent Chip, each column is independently examined, and a Performance Chromatogram and Certificate of Batch Analysis are provided. The Performance Test Chromatogram is specific to each lab equipment and contains information such as the gel lot batch number, column serial number, USP plate count, USP tailing factor, capacity factor as well as chromatographic results and conditions. It is highly recommended that these data should be stored for future reference.
The BioSuite brand offers other varieties and columns for your chromatographic needs. You can explore the entire range and shop for lab equipment using our website. You may, for instance, want to learn more about the BioSuite C18 PA-A Column, 100Å, 3 µm, 4.6 mm X 50 mm, 1K - 15K, 1/pk.
What Are The Minimum And Maximum Molecular Weights That The BioSuite C18 PA-B Column Can Process?
The BioSuite C18 PA-B Column listed here can process a minimum molecular weight of 1000 and a maximum of 30,000.
What Is The Carbon Load In The BioSuite C18 PA-B Column?
This particular BioSuite C18 PA-B Column has a carbon load of 8.5 %.