Primarily supports legacy methods. BioSuite Peptide Analysis columns consist of two premier reversed-phase column chemistries specifically optimized for peptide mapping from simple to complicated digests. BioSuite PA Columns are available in various configurations for LC or LC-MS applications, including 250 mm lengths for ultra high resolution. These BioSuite PA-A and PA-B columns provide excellent batch-to-batch reproducibility for consistent results and are uniquely QC tested specifically for peptide mapping. BioSuite C18 3 µm PA-A is a 100Å, difunctional bonded, low ligand density, silica-based reversed-phase column and BioSuite C18 3.5 µm PA-B is a 300Å, high ligand density, monofunctional, silica-based reversed-phase column.
Chemistry |
C18 |
Separation Mode |
Reversed Phase |
Particle Substrate |
Silica |
pH Range Min |
2 pH |
pH Range Max |
8 pH |
Maximum Pressure |
6000 psi (415 Bar) |
Endcapped |
Yes |
Molecular Weight Range Min |
1000 |
Molecular Weight Range Max |
30000 |
Particle Shape |
Spherical |
Particle Size |
3 µm |
Endfitting Type |
Waters |
Pore Size |
100 Å |
Format |
Column |
System |
HPLC |
USP Classification |
L1 |
Inner Diameter |
4.6 mm |
Length |
250 mm |
Carbon Load |
8.5 % |
UNSPSC |
41115709 |
Application |
Peptide |
Brand |
BioSuite |
Product Type |
Columns |
Units per Package |
1 pk |
BioSuite C18 PA-A Column, 100Å, 3 µm, 4.6 mm x 250 mm, 1K - 15K, 1/pk
The BioSuite C18 PA-A Column is a dielectrically bonded silica-based column with a low ligand density. It has been precisely developed for outstanding polar peptide retention, making it an excellent choice for use as a column in LC-MS applications that use formic acid (FA) to reduce ion suppression. TFA concentrations of 0.025 percent TFA work well with the BioSuite C18 PA-A Column in traditional HPLC separations.
BioSuite™ Peptide Analysis columns consist of two premier reversed-phase column chemistries specifically optimized for peptide mapping from simple to complicated digests. These BioSuite™ PA-A and PA-B columns provide excellent batch-to-batch reproducibility for consistent results and are uniquely QC tested specifically for peptide mapping. BioSuite™ C18 3µm PA-A is a100Å, difunctional bonded, low ligand density, silica-based reverse-phase column and BioSuite™ C18 3.5µm PA-B is a 300Å, high ligand density, monofunctional, silica-based reverse-phase column.
In a dedicated Waters chromatography chemistry production unit, the BioSuite C18 PA-A Column column is made under the strictest cGMP, ISO 9002 conditions. At the manufacturing facility, silica-based and polymeric chromatographic materials are made and packed into lab equipment and solid-phase extraction devices. This guarantees that you will receive top-of-the-line lab equipment that will deliver consistent and reliable analysis results.
BioSuite PA Columns are available in various configurations for LC or LC/MS applications, including 250 mm lengths for ultra-high resolution. You can explore the complete range using the Waters website, which contains details of each product available in our portfolio and also enables you to shop for lab equipment.
We recommend that you also invest in the Deactivated Clear Glass 12 x 32 mm Screw Neck Vial, with Cap and PTFE/Silicone Septum, 2 mL Volume, 100/pk. When working with biological or pharmaceutical substances, natural goods, insecticides, and herbicides, the vial has been specifically coated to prevent compound absorption onto the glass surface. Glass vials are also treated with a gas-phase reactive organosilane to create a hydrophobic glass surface that is compatible with glass-sensitive chemicals, as well as making it chemically inert and eliminating the possibility of it interacting with your product.
What pH Is Suited To The BioSuite C18 PA-A Column?
This particular BioSuite C18 PA-A Column is able to work well across the 2-8 pH range.
What Is The Maximum Pressure That The BioSuite C18 PA-A Column Can Sustain?
The BioSuite C18 PA-A Column listed here is able to work well with a maximum of 6000 psi (415 Bar) pressure.