Primarily supports legacy methods. BioSuite SEC Columns are available in 4.6 mm, 7.5 mm, and 7.8 mm i.d. analytical and 21.5 mm i.d. preparative and guard columns.
Chemistry |
Diol (OH) |
Separation Mode |
SEC |
Particle Substrate |
Silica |
pH Range Min |
2.5 pH |
pH Range Max |
7.5 pH |
Maximum Pressure |
150 psi (10 bar) |
Endcapped |
Yes |
Molecular Weight Range Min |
5000 |
Molecular Weight Range Max |
150000 |
Particle Shape |
Spherical |
Particle Size |
13 µm |
Endfitting Type |
Parker-style |
Pore Size |
125 Å |
Format |
Column |
System |
HPLC |
USP Classification |
L59 |
Inner Diameter |
21.5 mm |
Length |
300 mm |
UNSPSC |
41115709 |
Application |
Protein |
Brand |
BioSuite |
Product Type |
Columns |
Units per Package |
1 pk |
BioSuite Diol (OH) Column, 125Å, 13 µm, 21.5 mm X 300 mm, 5K - 150K, 1/pk
The BioSuite Diol (OH) Column is designed using High Resolution (HR) packing that uses a wettable silica-based material; this packing makes the column quite rigid and gives it chemical stability across a pH range of 2.5 to 7.5. With its length and particle size, the BioSuite Diol (OH) Column has increased separation efficiency.
The core design of the BioSuite Diol (OH) Column is focused on meeting the challenges of protein and peptide analysis. BioSuite Diol (OH) Column's chemistry makes it ideal to be used for isolation, analysis, and characterization of proteins and peptides. Given the demanding nature of the standards of the chromatographic analysis, each of the BioSuite Diol (OH) Columns goes through an extensive testing process to ensure product quality and batch-to-batch and column-to-column reproducibility. This particular BioSuite Diol (OH) Column is well suited for the analysis of spherical 13 µm particles in the molecular weight range of 5,000 - 150,000 Daltons.
BioSuite Diol (OH) Column will give you excellent resolution and recovery of proteins and peptides and yield superb results. You can rely on the BioSuite Diol (OH) Column to solve your protein separation and characterization challenges and produce successful protein-based biopharmaceuticals and diagnostic reagents by adequately characterizing complex biomolecules. This makes the BioSuite Diol (OH) Column a must-have piece of lab equipment as you can use the orthogonal separation techniques to produce the next-generation drug as the equipment helps to showcase critical characterization data and isolated materials required.
To learn more about the variation of the same product, such as the BioSuite Diol (OH) Column listed here, or to find different products that work with it or even to shop for lab equipment so you can replenish your stock and add to your existing equipment portfolio, you can browse our website and refer to our catalog.
You may also be interested in reviewing the BioSuite Diol (OH) Guard Column, 5 µm, 6 mm X 40 mm, 1/pk, which has been designed using the same packing material as the analytical column making it ideal to be used with it. The Guard Column ensures that any harmful chemical or particulate contaminants are removed from the mobile phase to protect your analytical column, thereby increasing its lifetime and efficiency.
How Is Protein Mass Calculated?
To determine the molecular weight of a protein, standard calculations involve summing up the individual molecular weights of its constituent amino acids. This approach allows for the accurate assessment of the protein's overall molecular mass. By assigning specific values to each amino acid and performing the summation, scientists can obtain valuable information about the size and composition of the protein. This fundamental calculation provides insights into the structural and functional characteristics of proteins, aiding in various fields such as biochemistry, molecular biology, and biotechnology.